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TitleMediator structure and conformation change.
Journal, issue, pagesMol Cell, Vol. 81, Issue 8, Page 1781-11788.e4, Year 2021
Publish dateApr 15, 2021
AuthorsHeqiao Zhang / Dong-Hua Chen / Rayees U H Mattoo / David A Bushnell / Yannan Wang / Chao Yuan / Lin Wang / Chunnian Wang / Ralph E Davis / Yan Nie / Roger D Kornberg /
PubMed AbstractMediator is a universal adaptor for transcription control. It serves as an interface between gene-specific activator or repressor proteins and the general RNA polymerase II (pol II) transcription ...Mediator is a universal adaptor for transcription control. It serves as an interface between gene-specific activator or repressor proteins and the general RNA polymerase II (pol II) transcription machinery. Previous structural studies revealed a relatively small part of Mediator and none of the gene activator-binding regions. We have determined the cryo-EM structure of the Mediator at near-atomic resolution. The structure reveals almost all amino acid residues in ordered regions, including the major targets of activator proteins, the Tail module, and the Med1 subunit of the Middle module. Comparison of Mediator structures with and without pol II reveals conformational changes that propagate across the entire Mediator, from Head to Tail, coupling activator- and pol II-interacting regions.
External linksMol Cell / PubMed:33571424
MethodsEM (single particle)
Resolution3.58 - 4.3 Å
Structure data

22284

6xp5

EMDB-22284, PDB-6xp5:
Head-Middle module of Mediator
Method: EM (single particle) / Resolution: 4.2 Å

22397

7jmn

EMDB-22397: T module
PDB-7jmn: Tail module of Mediator complex
Method: EM (single particle) / Resolution: 3.58 Å

EMDB-22399:
Mediator-Pol II complex
Method: EM (single particle) / Resolution: 4.3 Å

Source
  • chaetomium thermophilum (fungus)
  • chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719) (fungus)
  • chaetomium thermophilum var. thermophilum dsm 1495 (fungus)
KeywordsTRANSCRIPTION / Activation / PolII

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