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TitleStructural basis of copper-efflux-regulator-dependent transcription activation.
Journal, issue, pagesiScience, Vol. 24, Issue 5, Page 102449, Year 2021
Publish dateMay 21, 2021
AuthorsWei Shi / Baoyue Zhang / Yanan Jiang / Chang Liu / Wei Zhou / Ming Chen / Yang Yang / Yangbo Hu / Bin Liu /
PubMed AbstractThe copper efflux regulator (CueR), a representative member of mercury resistance regulator (MerR) family metalloregulators, controls expression of copper homeostasis-regulating genes in bacteria. ...The copper efflux regulator (CueR), a representative member of mercury resistance regulator (MerR) family metalloregulators, controls expression of copper homeostasis-regulating genes in bacteria. The mechanism of transcription activation by CueR and other MerR family regulators is bending the spacer domain of promoter DNA. Here, we report the cryo-EM structures of the intact CueR-dependent transcription activation complexes. The structures show that CueR dimer bends the 19-bp promoter spacer to realign the -35 and -10 elements for recognition by σ-RNA polymerase holoenzyme and reveal a previously unreported interaction between the DNA-binding domain (DBD) from one CueR subunit and the σ nonconserved region (σNCR). Functional studies have shown that the CueR-σNCR interaction plays an auxiliary role in CueR-dependent transcription, assisting the activation mechanism of bending promoter DNA by CueR dimer. Because DBDs are highly conserved in sequence and structure, this transcription-activating mechanism could be generally used by MerR family regulators.
External linksiScience / PubMed:34113812 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 4.1 Å
Structure data

22184

6xh7

EMDB-22184, PDB-6xh7:
CueR-TAC without RNA
Method: EM (single particle) / Resolution: 3.9 Å

22185

6xh8

EMDB-22185, PDB-6xh8:
CueR-transcription activation complex with RNA transcript
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-22289:
cross-reference map for CueR-TAC
Method: EM (single particle) / Resolution: 4.1 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-CU:
COPPER (II) ION

Source
  • escherichia coli (E. coli)
KeywordsTRANSCRIPTION/DNA / transcription activation / RNA polymerase / MerR-family / TRANSCRIPTION / TRANSCRIPTION-DNA complex / TRANSCRIPTION/DNA/RNA / TRANSCRIPTION-DNA-RNA complex

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