Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Dynamic and asymmetric fluctuations in the microtubule wall captured by high-resolution cryoelectron microscopy.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 117, Issue 29, Page 16976-16984, Year 2020
Publish date	Jul 21, 2020
Authors	Garrett E Debs / Michael Cha / Xueqi Liu / Andrew R Huehn / Charles V Sindelar /
PubMed Abstract	Microtubules are tubular polymers with essential roles in numerous cellular activities. Structures of microtubules have been captured at increasing resolution by cryo-EM. However, dynamic properties ...Microtubules are tubular polymers with essential roles in numerous cellular activities. Structures of microtubules have been captured at increasing resolution by cryo-EM. However, dynamic properties of the microtubule are key to its function, and this behavior has proved difficult to characterize at a structural level due to limitations in existing structure determination methods. We developed a high-resolution cryo-EM refinement method that divides an imaged microtubule into its constituent protofilaments, enabling deviations from helicity and other sources of heterogeneity to be quantified and corrected for at the single-subunit level. We demonstrate that this method improves the resolution of microtubule 3D reconstructions and substantially reduces anisotropic blurring artifacts, compared with methods that utilize helical symmetry averaging. Moreover, we identified an unexpected, discrete behavior of the m-loop, which mediates lateral interactions between neighboring protofilaments and acts as a flexible hinge between them. The hinge angle adopts preferred values corresponding to distinct conformations of the m-loop that are incompatible with helical symmetry. These hinge angles fluctuate in a stochastic manner, and perfectly cylindrical microtubule conformations are thus energetically and entropically penalized. The hinge angle can diverge further from helical symmetry at the microtubule seam, generating a subpopulation of highly distorted microtubules. However, the seam-distorted subpopulation disappears in the presence of Taxol, a microtubule stabilizing agent. These observations provide clues into the structural origins of microtubule flexibility and dynamics and highlight the role of structural polymorphism in defining microtubule behavior.
External links	Proc Natl Acad Sci U S A / PubMed:32636254 / PubMed Central
Methods	EM (single particle)
Resolution	2.9 - 3.6 Å
Structure data	EMDB-21919: 14-protofilament, GMPCPP stabilized microtubule with nucleotide free kinesin Method: EM (single particle) / Resolution: 3.6 Å 21922 6wvl EMDB-21922, PDB-6wvl: Low curvature lateral interaction within a 13-protofilament, Taxol stabilized microtubule Method: EM (single particle) / Resolution: 3.2 Å 21923 6wvm EMDB-21923, PDB-6wvm: High curvature lateral interaction within a 13-protofilament, Taxol stabilized microtubule Method: EM (single particle) / Resolution: 3.3 Å 21924 6wvr EMDB-21924: Protofilament from a 13-protofilament, Taxol stabilized microtubule PDB-6wvr: Tubulin dimers from a 13-protofilament, Taxol stabilized microtubule Method: EM (single particle) / Resolution: 2.9 Å
Chemicals	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM / Guanosine triphosphate ChemComp-MG: Unknown entry ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying moleculeYM / Guanosine diphosphate ChemComp-TA1: TAXOL / medication, chemotherapy*YM / Paclitaxel
Source	bos taurus (cattle)
Keywords	STRUCTURAL PROTEIN / Microtubule