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TitleMammalian Retromer Is an Adaptable Scaffold for Cargo Sorting from Endosomes.
Journal, issue, pagesStructure, Vol. 28, Issue 4, Page 393-405.e4, Year 2020
Publish dateApr 7, 2020
AuthorsAmy K Kendall / Boyang Xie / Peng Xu / Jue Wang / Rodger Burcham / Meredith N Frazier / Elad Binshtein / Hui Wei / Todd R Graham / Terunaga Nakagawa / Lauren P Jackson /
PubMed AbstractMetazoan retromer (VPS26/VPS35/VPS29) associates with sorting nexins on endosomal tubules to sort proteins to the trans-Golgi network or plasma membrane. Mechanisms of metazoan retromer assembly ...Metazoan retromer (VPS26/VPS35/VPS29) associates with sorting nexins on endosomal tubules to sort proteins to the trans-Golgi network or plasma membrane. Mechanisms of metazoan retromer assembly remain undefined. We combine single-particle cryoelectron microscopy with biophysical methods to uncover multiple oligomer structures. 2D class averages reveal mammalian heterotrimers; dimers of trimers; tetramers of trimers; and flat chains. These species are further supported by biophysical solution studies. We provide reconstructions of all species, including key sub-structures (∼5 Å resolution). Local resolution variation suggests that heterotrimers and dimers adopt multiple conformations. Our structures identify a flexible, highly conserved electrostatic dimeric interface formed by VPS35 subunits. We generate structure-based mutants to disrupt this interface in vitro. Equivalent mutations in yeast demonstrate a mild cargo-sorting defect. Our data suggest the metazoan retromer is an adaptable and plastic scaffold that accommodates interactions with different sorting nexins to sort multiple cargoes from endosomes their final destinations.
External linksStructure / PubMed:32027819 / PubMed Central
MethodsEM (single particle)
Resolution4.9 - 25.5 Å
Structure data

EMDB-21101:
Mouse retromer (VPS26/VPS35/VPS29) tetramer of heterotrimers
Method: EM (single particle) / Resolution: 25.5 Å

EMDB-21116:
Mouse retromer (VPS26/VPS35/VPS29) chain interface I (VPS35/VPS35 dimer)
Method: EM (single particle) / Resolution: 6.9 Å

EMDB-21117:
Mouse retromer (VPS26/VPS35/VPS29) dimer of heterotrimers
Method: EM (single particle) / Resolution: 9.3 Å

EMDB-21118:
Mouse retromer (VPS26/VPS35/VPS29) chain interface II (VPS26/VPS26 dimer)
Method: EM (single particle) / Resolution: 16.9 Å

EMDB-21119:
Mouse retromer sub-structure: VPS35/VPS35 curved dimer
Method: EM (single particle) / Resolution: 5.3 Å

21135

6vab

EMDB-21135, PDB-6vab:
Mouse retromer sub-structure: VPS35/VPS35 flat dimer
Method: EM (single particle) / Resolution: 4.9 Å

21136

6vac

EMDB-21136, PDB-6vac:
Mouse retromer (VPS26/VPS35/VPS29) heterotrimer
Method: EM (single particle) / Resolution: 5.7 Å

Source
  • mus musculus (house mouse)
KeywordsPROTEIN TRANSPORT / retromer / membrane trafficking / endosomal trafficking / membrane coat complexes

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