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TitleStructure of an active human histone pre-mRNA 3'-end processing machinery.
Journal, issue, pagesScience, Vol. 367, Issue 6478, Page 700-703, Year 2020
Publish dateFeb 7, 2020
AuthorsYadong Sun / Yixiao Zhang / Wei Shen Aik / Xiao-Cui Yang / William F Marzluff / Thomas Walz / Zbigniew Dominski / Liang Tong /
PubMed AbstractThe 3'-end processing machinery for metazoan replication-dependent histone precursor messenger RNAs (pre-mRNAs) contains the U7 small nuclear ribonucleoprotein and shares the key cleavage module with ...The 3'-end processing machinery for metazoan replication-dependent histone precursor messenger RNAs (pre-mRNAs) contains the U7 small nuclear ribonucleoprotein and shares the key cleavage module with the canonical cleavage and polyadenylation machinery. We reconstituted an active human histone pre-mRNA processing machinery using 13 recombinant proteins and two RNAs and determined its structure by cryo-electron microscopy. The overall structure is highly asymmetrical and resembles an amphora with one long handle. We captured the pre-mRNA in the active site of the endonuclease, the 73-kilodalton subunit of the cleavage and polyadenylation specificity factor, poised for cleavage. The endonuclease and the entire cleavage module undergo extensive rearrangements for activation, triggered through the recognition of the duplex between the authentic pre-mRNA and U7 small nuclear RNA (snRNA). Our study also has notable implications for understanding canonical and snRNA 3'-end processing.
External linksScience / PubMed:32029631 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 4.7 Å
Structure data

EMDB-21045:
Cryo-EM map of human symplekin CTD
Method: EM (single particle) / Resolution: 4.7 Å

EMDB-21046:
Cryo-EM map of the core of human histone pre-mRNA cleavage complex (CPSF73-CPSF100-Symplekin)
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-21047:
Cryo-EM map of the overall structure of human histone pre-mRNA 3'-end processing machinery
Method: EM (single particle) / Resolution: 4.1 Å

21050

6v4x

EMDB-21050, PDB-6v4x:
Cryo-EM structure of an active human histone pre-mRNA 3'-end processing machinery at 3.2 Angstrom resolution
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
KeywordsRNA BINDING PROTEIN/RNA / endonuclease / active CPSF73 / U7 snRNP / 3'-end processing / RIBONUCLEASE / RNA BINDING PROTEIN-RNA complex

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