Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development.
Journal, issue, pages	mBio, Vol. 11, Issue 1, Year 2020
Publish date	Jan 21, 2020
Authors	Christopher E Morgan / Wei Huang / Susan D Rudin / Derek J Taylor / James E Kirby / Robert A Bonomo / Edward W Yu /
PubMed Abstract	Antimicrobial resistance is a major health threat as it limits treatment options for infection. At the forefront of this serious issue is , a Gram-negative opportunistic pathogen that exhibits the ...Antimicrobial resistance is a major health threat as it limits treatment options for infection. At the forefront of this serious issue is , a Gram-negative opportunistic pathogen that exhibits the remarkable ability to resist antibiotics through multiple mechanisms. As bacterial ribosomes represent a target for multiple distinct classes of existing antimicrobial agents, we here use single-particle cryo-electron microscopy (cryo-EM) to elucidate five different structural states of the ribosome, including the 70S, 50S, and 30S forms. We also determined interparticle motions of the 70S ribosome in different tRNA bound states using three-dimensional (3D) variability analysis. Together, our structural data further our understanding of the ribosome from and other Gram-negative pathogens and will enable structure-based drug discovery to combat antibiotic-resistant bacterial infections. is a severe nosocomial threat largely due to its intrinsic antibiotic resistance and remarkable ability to acquire new resistance determinants. The bacterial ribosome serves as a major target for modern antibiotics and the design of new therapeutics. Here, we present cryo-EM structures of the 70S ribosome, revealing several unique species-specific structural features that may facilitate future drug development to combat this recalcitrant bacterial pathogen.
External links	mBio / PubMed:31964740 / PubMed Central
Methods	EM (single particle)
Resolution	2.82 - 4.4 Å
Structure data	21030 6v39 EMDB-21030, PDB-6v39: Cryo-EM structure of the Acinetobacter baumannii Ribosome: 70S with P-site tRNA Method: EM (single particle) / Resolution: 3.04 Å 21031 6v3a EMDB-21031, PDB-6v3a: Cryo-EM structure of the Acinetobacter baumannii Ribosome: 70S with E-site tRNA Method: EM (single particle) / Resolution: 2.82 Å 21032 6v3b EMDB-21032, PDB-6v3b: Cryo-EM structure of the Acinetobacter baumannii Ribosome: 70S in Empty state Method: EM (single particle) / Resolution: 2.91 Å 21033 6v3d EMDB-21033, PDB-6v3d: Cryo-EM structure of the Acinetobacter baumannii Ribosome: 50S subunit Method: EM (single particle) / Resolution: 2.95 Å 21034 6v3e EMDB-21034, PDB-6v3e: Cryo-EM structure of the Acinetobacter baumannii Ribosome: 30S subunit Method: EM (single particle) / Resolution: 4.4 Å
Chemicals	ChemComp-ZN: Unknown entry ChemComp-FME: N-FORMYLMETHIONINE ChemComp-MG: Unknown entry ChemComp-NA: Unknown entry ChemComp-HOH: WATER
Source	acinetobacter baumannii ab0057 (bacteria) acinetobacter sp. cip 51.11 (bacteria) acinetobacter beijerinckii anc 3835 (bacteria) acinetobacter baumannii (strain ab0057) (bacteria) acinetobacter sp. anc 4470 (bacteria) acinetobacter sp. niph 899 (bacteria) acinetobacter baumannii (bacteria) acinetobacter sp. 809848 (bacteria) acinetobacter rudis cip 110305 (bacteria) acinetobacter sp. cip 102082 (bacteria) acinetobacter sp. anc 5600 (bacteria) acinetobacter sp. 263903-1 (bacteria) acinetobacter venetianus (bacteria)
Keywords	RIBOSOME / Acinetobacter baumannii