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TitleStructure of the Vesicular Stomatitis Virus L Protein in Complex with Its Phosphoprotein Cofactor.
Journal, issue, pagesCell Rep, Vol. 30, Issue 1, Page 53-60.e5, Year 2020
Publish dateJan 7, 2020
AuthorsSimon Jenni / Louis-Marie Bloyet / Ruben Diaz-Avalos / Bo Liang / Sean P J Whelan / Nikolaus Grigorieff / Stephen C Harrison /
PubMed AbstractThe large (L) proteins of non-segmented, negative-strand RNA viruses are multifunctional enzymes that produce capped, methylated, and polyadenylated mRNA and replicate the viral genome. A ...The large (L) proteins of non-segmented, negative-strand RNA viruses are multifunctional enzymes that produce capped, methylated, and polyadenylated mRNA and replicate the viral genome. A phosphoprotein (P), required for efficient RNA-dependent RNA polymerization from the viral ribonucleoprotein (RNP) template, regulates the function and conformation of the L protein. We report the structure of vesicular stomatitis virus L in complex with its P cofactor determined by electron cryomicroscopy at 3.0 Å resolution, enabling us to visualize bound segments of P. The contacts of three P segments with multiple L domains show how P induces a closed, compact, initiation-competent conformation. Binding of P to L positions its N-terminal domain adjacent to a putative RNA exit channel for efficient encapsidation of newly synthesized genomes with the nucleoprotein and orients its C-terminal domain to interact with an RNP template. The model shows that a conserved tryptophan in the priming loop can support the initiating 5' nucleotide.
External linksCell Rep / PubMed:31914397 / PubMed Central
MethodsEM (single particle)
Resolution3.0 Å
Structure data

20614

6u1x

EMDB-20614, PDB-6u1x:
Structure of the Vesicular Stomatitis Virus L Protein in Complex with Its Phosphoprotein Cofactor (3.0 A resolution)
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • Vesicular stomatitis virus
  • vesicular stomatitis indiana virus (strain san juan)
KeywordsVIRAL PROTEIN / Vesicular stomatitis virus / RNA-dependent RNA polymerase / L protein / P protein / phosphoprotein / single particle analysis / transcription / replication / virus / viral / RdRp / PRNTase / nonsegmented negative-sense RNA viruses

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