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Structure paper

TitleTubulin lattice in cilia is in a stressed form regulated by microtubule inner proteins.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 116, Issue 40, Page 19930-19938, Year 2019
Publish dateOct 1, 2019
AuthorsMuneyoshi Ichikawa / Ahmad Abdelzaher Zaki Khalifa / Shintaroh Kubo / Daniel Dai / Kaustuv Basu / Mohammad Amin Faghfor Maghrebi / Javier Vargas / Khanh Huy Bui /
PubMed AbstractCilia, the hair-like protrusions that beat at high frequencies to propel a cell or move fluid around are composed of radially bundled doublet microtubules. In this study, we present a near-atomic ...Cilia, the hair-like protrusions that beat at high frequencies to propel a cell or move fluid around are composed of radially bundled doublet microtubules. In this study, we present a near-atomic resolution map of the doublet microtubule by cryoelectron microscopy. The map demonstrates that the network of microtubule inner proteins weaves into the tubulin lattice and forms an inner sheath. From mass spectrometry data and de novo modeling, we identified Rib43a proteins as the filamentous microtubule inner proteins in the protofilament ribbon region. The Rib43a-tubulin interaction leads to an elongated tubulin dimer distance every 2 dimers. In addition, the tubulin lattice structure with missing microtubule inner proteins (MIPs) by sarkosyl treatment shows significant longitudinal compaction and lateral angle change between protofilaments. These results are evidence that the MIPs directly affect and stabilize the tubulin lattice. It suggests that the doublet microtubule is an intrinsically stressed filament and that this stress could be manipulated in the regulation of ciliary waveforms.
External linksProc Natl Acad Sci U S A / PubMed:31527277 / PubMed Central
MethodsEM (single particle)
Resolution4.16 - 4.9 Å
Structure data

20602

6u0h

6u0t

6u0u

EMDB-20602: Cryo-EM structure of the 48-nm repeat unit of the doublet microtubule from Tetrahymena thermophila
PDB-6u0h: Tubulin lattice of the ciliary doublet microtubule from Tetrahymena thermophila
PDB-6u0t: Protofilament Ribbon Flagellar Proteins Rib43a-S
PDB-6u0u: Protofilament Ribbon Flagellar Proteins Rib43a-L
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-20603:
Cryo-EM map of the A-tubule prepared by sonication
Method: EM (single particle) / Resolution: 4.4 Å

EMDB-20606:
Cryo-EM map of the A-tubule prepared by 0.2% Sarkosyl treatment
Method: EM (single particle) / Resolution: 4.9 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Source
  • tetrahymena thermophila (eukaryote)
  • tetrahymena thermophila (strain sb210) (eukaryote)
KeywordsSTRUCTURAL PROTEIN / cilia / doublet / axoneme / microtubule inner protein / ribbon proteins

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