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TitleStructure-function analysis of the cyclic β-1,2-glucan synthase from Agrobacterium tumefaciens.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 1844, Year 2024
Publish dateFeb 28, 2024
AuthorsJaroslaw Sedzicki / Dongchun Ni / Frank Lehmann / Henning Stahlberg / Christoph Dehio /
PubMed AbstractThe synthesis of complex sugars is a key aspect of microbial biology. Cyclic β-1,2-glucan (CβG) is a circular polysaccharide critical for host interactions of many bacteria, including major ...The synthesis of complex sugars is a key aspect of microbial biology. Cyclic β-1,2-glucan (CβG) is a circular polysaccharide critical for host interactions of many bacteria, including major pathogens of humans (Brucella) and plants (Agrobacterium). CβG is produced by the cyclic glucan synthase (Cgs), a multi-domain membrane protein. So far, its structure as well as the mechanism underlining the synthesis have not been clarified. Here we use cryo-electron microscopy (cryo-EM) and functional approaches to study Cgs from A. tumefaciens. We determine the structure of this complex protein machinery and clarify key aspects of CβG synthesis, revealing a distinct mechanism that uses a tyrosine-linked oligosaccharide intermediate in cycles of polymerization and processing of the glucan chain. Our research opens possibilities for combating pathogens that rely on polysaccharide virulence factors and may lead to synthetic biology approaches for producing complex cyclic sugars.
External linksNat Commun / PubMed:38418509 / PubMed Central
MethodsEM (single particle)
Resolution3.28 - 5.99 Å
Structure data

19114

8rf0

EMDB-19114, PDB-8rf0:
WT-CGS sample in nanodisc
Method: EM (single particle) / Resolution: 3.4 Å

19116

8rf9

EMDB-19116, PDB-8rf9:
CgsiGP1 sample in nanodisc
Method: EM (single particle) / Resolution: 3.28 Å

19118

8rfe

EMDB-19118, PDB-8rfe:
CgsiGP2 sample in nanodisc
Method: EM (single particle) / Resolution: 3.8 Å

19119

8rfg

EMDB-19119, PDB-8rfg:
CgsiGP3 sample in nanodisc
Method: EM (single particle) / Resolution: 3.35 Å

EMDB-19123: cyclic b-1,2-glucan synthase IGT mutant
Method: EM (single particle) / Resolution: 5.99 Å

Chemicals

ChemComp-UPG:
URIDINE-5'-DIPHOSPHATE-GLUCOSE

ChemComp-HOH:
WATER

Source
  • agrobacterium tumefaciens (bacteria)
KeywordsANTIBIOTIC / cgs / cyclic glucan

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