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TitleStructure and replication cycle of a virus infecting climate-modulating alga .
Journal, issue, pagesSci Adv, Vol. 10, Issue 15, Page eadk1954, Year 2024
Publish dateApr 12, 2024
AuthorsMiroslav Homola / Carina R Büttner / Tibor Füzik / Pavel Křepelka / Radka Holbová / Jiří Nováček / Marten L Chaillet / Jakub Žák / Danyil Grybchuk / Friedrich Förster / William H Wilson / Declan C Schroeder / Pavel Plevka /
PubMed AbstractThe globally distributed marine alga has cooling effect on the Earth's climate. The population density of is restricted by viruses, including virus 201 (EhV-201). Despite the impact of viruses ...The globally distributed marine alga has cooling effect on the Earth's climate. The population density of is restricted by viruses, including virus 201 (EhV-201). Despite the impact of viruses on the climate, there is limited information about their structure and replication. Here, we show that the dsDNA genome inside the EhV-201 virion is protected by an inner membrane, capsid, and outer membrane. EhV-201 virions infect by using fivefold vertices to bind to and fuse the virus' inner membrane with the cell plasma membrane. Progeny virions assemble in the cytoplasm at the surface of endoplasmic reticulum-derived membrane segments. Genome packaging initiates synchronously with the capsid assembly and completes through an aperture in the forming capsid. The genome-filled capsids acquire an outer membrane by budding into intracellular vesicles. EhV-201 infection induces a loss of surface protective layers from cells, which enables the continuous release of virions by exocytosis.
External linksSci Adv / PubMed:38598627 / PubMed Central
MethodsEM (subtomogram averaging) / EM (single particle) / EM (tomography)
Resolution12.0 - 25.0 Å
Structure data

EMDB-17649: Cryo-EM structure of the Emiliania huxleyi virus 201 (EhV-201) virion vertex with a diameter of 50 nm.
Method: EM (subtomogram averaging) / Resolution: 13.0 Å

EMDB-17650: Cryo-EM structure of the Emiliania huxleyi virus 201 (EhV-201) virion vertex with a diameter of 50 nm.
Method: EM (single particle) / Resolution: 25.0 Å

EMDB-17651: Cryo-EM structure of the Emiliania huxleyi virus 201 (EhV-201) virion vertex with a diameter of 120 nm.
Method: EM (subtomogram averaging) / Resolution: 18.0 Å

EMDB-17823: Tomogram of the Emiliania huxleyi virus 201 (EhV-201) purified particles used for subtomogram averaging.
Method: EM (tomography) / Resolution: 25.0 Å

19035

8rbs

EMDB-19035: Composite map of the Emiliania huxleyi virus 201 (EhV-201) symmetry expanded from cryo-EM structure of virion vertex 120 nm in diameter.
PDB-8rbs: Emiliania huxleyi virus 201 (EhV-201) asymmetrical unit of capsid proteins predicted by AlphaFold2 fitted into the cryo-EM density of EhV-201 virion composite map.
Method: EM (subtomogram averaging) / Resolution: 18.0 Å

19036

8rbt

EMDB-19036: Cryo-EM structure of the Emiliania huxleyi virus 201 (EhV-201) virion vertex with a diameter of 50 nm and a mask applied on the capsid layer.
PDB-8rbt: Emiliania huxleyi virus 201 (EhV-201) capsid proteins predicted by AlphaFold2 fitted into a cryo-EM density map of the EhV-201 virion capsid.
Method: EM (subtomogram averaging) / Resolution: 12.0 Å

Source
  • emiliania huxleyi virus 201
KeywordsVIRUS / cryo-EM / subtomogram averaging / EhV-201 / enveloped virus / capsid / major capsid protein / composite map

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