[English] 日本語
Yorodumi- PDB-8rbs: Emiliania huxleyi virus 201 (EhV-201) asymmetrical unit of capsid... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8rbs | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Emiliania huxleyi virus 201 (EhV-201) asymmetrical unit of capsid proteins predicted by AlphaFold2 fitted into the cryo-EM density of EhV-201 virion composite map. | |||||||||
Components |
| |||||||||
Keywords | VIRUS / cryo-EM / subtomogram averaging / EhV-201 / enveloped virus / capsid / major capsid protein / composite map | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Emiliania huxleyi virus 201 | |||||||||
Method | ELECTRON MICROSCOPY / subtomogram averaging / cryo EM / Resolution: 18 Å | |||||||||
Authors | Homola, M. / Buttner, C.R. / Fuzik, T. / Novacek, J. / Chaillet, M. / Forster, F. / Plevka, P. | |||||||||
Funding support | Czech Republic, European Union, 2items
| |||||||||
Citation | Journal: Sci Adv / Year: 2024 Title: Structure and replication cycle of a virus infecting climate-modulating alga . Authors: Miroslav Homola / Carina R Büttner / Tibor Füzik / Pavel Křepelka / Radka Holbová / Jiří Nováček / Marten L Chaillet / Jakub Žák / Danyil Grybchuk / Friedrich Förster / William H ...Authors: Miroslav Homola / Carina R Büttner / Tibor Füzik / Pavel Křepelka / Radka Holbová / Jiří Nováček / Marten L Chaillet / Jakub Žák / Danyil Grybchuk / Friedrich Förster / William H Wilson / Declan C Schroeder / Pavel Plevka / Abstract: The globally distributed marine alga has cooling effect on the Earth's climate. The population density of is restricted by viruses, including virus 201 (EhV-201). Despite the impact of viruses ...The globally distributed marine alga has cooling effect on the Earth's climate. The population density of is restricted by viruses, including virus 201 (EhV-201). Despite the impact of viruses on the climate, there is limited information about their structure and replication. Here, we show that the dsDNA genome inside the EhV-201 virion is protected by an inner membrane, capsid, and outer membrane. EhV-201 virions infect by using fivefold vertices to bind to and fuse the virus' inner membrane with the cell plasma membrane. Progeny virions assemble in the cytoplasm at the surface of endoplasmic reticulum-derived membrane segments. Genome packaging initiates synchronously with the capsid assembly and completes through an aperture in the forming capsid. The genome-filled capsids acquire an outer membrane by budding into intracellular vesicles. EhV-201 infection induces a loss of surface protective layers from cells, which enables the continuous release of virions by exocytosis. | |||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 8rbs.cif.gz | 4.7 MB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb8rbs.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 8rbs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rb/8rbs ftp://data.pdbj.org/pub/pdb/validation_reports/rb/8rbs | HTTPS FTP |
---|
-Related structure data
Related structure data | 19035MC 8rbtC C: citing same article (ref.) M: map data used to model this data |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
|
---|---|
1 |
| x 60
-Components
#1: Protein | Mass: 55145.770 Da / Num. of mol.: 84 / Source method: isolated from a natural source / Details: AlphaFold2 predicted structure. / Source: (natural) Emiliania huxleyi virus 201 / References: UniProt: G9E4T6 #2: Protein | | Mass: 72841.266 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: AlphaFold2 predicted structure. / Source: (natural) Emiliania huxleyi virus 201 / References: UniProt: Q4A2T2 |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: subtomogram averaging |
-Sample preparation
Component | Name: Emiliania huxleyi virus 201 / Type: VIRUS Details: EhV-201 was propagated on a non-calcifying Emiliania huxleyi strain (CCPM 2090). Entity ID: all / Source: NATURAL | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Molecular weight | Experimental value: NO | ||||||||||||||||||||
Source (natural) | Organism: Emiliania huxleyi virus 201 | ||||||||||||||||||||
Details of virus | Empty: NO / Enveloped: YES / Isolate: SPECIES / Type: VIRION | ||||||||||||||||||||
Natural host | Organism: Emiliania huxleyi CCMP1516 / Strain: CCMP 2090 | ||||||||||||||||||||
Virus shell |
| ||||||||||||||||||||
Buffer solution | pH: 8 Details: Sea salts (Sigma Aldrich - S9883) were dissolved in distilled water (40 g/L w/v), filtered through a 0.22 um filter, and pH adjusted to 8. | ||||||||||||||||||||
Buffer component | Units: 40 / Name: sea salt | ||||||||||||||||||||
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES Details: The viral sample was concentrated down to 1x10^10 plaque-forming units per ml (PFU ml^-1) | ||||||||||||||||||||
Specimen support | Details: top side only / Grid material: COPPER / Grid mesh size: 200 divisions/in. / Grid type: Quantifoil R2/1 | ||||||||||||||||||||
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 283.15 K Details: Sample: 3.5 ul; Wait time: 10 s; Blot time: 3 s; Blot force: -2; Drain time: 0 s |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 42000 X / Calibrated magnification: 42000 X / Nominal defocus max: 4000 nm / Nominal defocus min: 2000 nm / Calibrated defocus min: 2000 nm / Calibrated defocus max: 4000 nm / Cs: 2.7 mm / C2 aperture diameter: 30 µm / Alignment procedure: COMA FREE |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Temperature (max): 77 K / Temperature (min): 77 K |
Image recording | Average exposure time: 1.5 sec. / Electron dose: 2.42 e/Å2 / Avg electron dose per subtomogram: 80 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Num. of real images: 4323 |
EM imaging optics | Energyfilter name: GIF Bioquantum / Energyfilter slit width: 10 eV |
Image scans | Sampling size: 5 µm / Width: 5760 / Height: 4092 |
-Processing
EM software |
| ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||||||||
Symmetry | Point symmetry: I (icosahedral) | ||||||||||||||||||||||||||||||||||||
3D reconstruction | Resolution: 18 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 3152 / Algorithm: FOURIER SPACE / Num. of class averages: 2 / Symmetry type: POINT | ||||||||||||||||||||||||||||||||||||
EM volume selection | Method: template matching / Num. of tomograms: 131 / Num. of volumes extracted: 10240 Reference model: EhV-201 virion vertex reconstruction coming out of localized single particle reconstruction | ||||||||||||||||||||||||||||||||||||
Atomic model building | Protocol: RIGID BODY FIT / Space: REAL / Target criteria: Cross-correlation coefficient | ||||||||||||||||||||||||||||||||||||
Atomic model building |
|