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Structure paper

TitleStructure of native photosystem II assembly intermediate from .
Journal, issue, pagesFront Plant Sci, Vol. 14, Page 1334608, Year 2023
Publish dateJan 23, 2024
AuthorsMariia Fadeeva / Daniel Klaiman / Eaazhisai Kandiah / Nathan Nelson /
PubMed Abstract Photosystem II (PSII) is a dimer consisting of at least 13 nuclear-encoded and four chloroplast-encoded protein subunits that collectively function as a sunlight-driven oxidoreductase. In this ... Photosystem II (PSII) is a dimer consisting of at least 13 nuclear-encoded and four chloroplast-encoded protein subunits that collectively function as a sunlight-driven oxidoreductase. In this study, we present the inaugural structure of a green alga PSII assembly intermediate (pre-PSII-int). This intermediate was isolated from chloroplast membranes of the temperature-sensitive mutant TSP4, cultivated for 14 hours at a non-permissive temperature. The assembly state comprises a monomer containing subunits A, B, C, D, E, F, H, I, K, and two novel assembly factors, Psb1 and Psb2. Psb1 is identified as a novel transmembrane helix located adjacent to PsbE and PsbF (cytochrome b559). The absence of PsbJ, typically found in mature PSII close to this position, indicates that Psb1 functions as an assembly factor. Psb2 is an eukaryotic homolog of the cyanobacterial assembly factor Psb27. The presence of iron, coupled with the absence of Q, Q, and the manganese cluster, implies a protective mechanism against photodamage and provides insights into the intricate assembly process.
External linksFront Plant Sci / PubMed:38322422 / PubMed Central
MethodsEM (single particle)
Resolution2.9 Å
Structure data

18848

8r2i

EMDB-18848: Cryo-EM Structure of native Photosystem II assembly intermediate fromChlamydomonas reinhardtii
PDB-8r2i: Cryo-EM Structure of native Photosystem II assembly intermediate from Chlamydomonas reinhardtii
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-FE2:
Unknown entry

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-PHO:
PHEOPHYTIN A / Pheophytin

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

ChemComp-BOG:
octyl beta-D-glucopyranoside / detergent*YM / Octyl glucoside

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM / Bicarbonate

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE / Plastoquinone

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

ChemComp-HOH:
WATER / Water

Source
  • chlamydomonas reinhardtii (plant)
KeywordsPHOTOSYNTHESIS / Photosystem II / biogenesis of PSII / assembly intermediate / CryoEM / assembly factor / Chlamydomonas reinhardtii

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