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-Structure paper
| Title | Nucleocapsid assembly drives Ebola viral factory maturation and dispersion. |
|---|---|
| Journal, issue, pages | Cell, Vol. 188, Issue 3, Page 704-720.e17, Year 2025 |
| Publish date | Feb 6, 2025 |
 Authors | Melina Vallbracht / Bianca S Bodmer / Konstantin Fischer / Jana Makroczyova / Sophie L Winter / Lisa Wendt / Moritz Wachsmuth-Melm / Thomas Hoenen / Petr Chlanda /  |
| PubMed Abstract | Replication and genome encapsidation of many negative-sense RNA viruses take place in virus-induced membraneless organelles termed viral factories (VFs). Although liquid properties of VFs are ...Replication and genome encapsidation of many negative-sense RNA viruses take place in virus-induced membraneless organelles termed viral factories (VFs). Although liquid properties of VFs are believed to control the transition from genome replication to nucleocapsid (NC) assembly, VF maturation and interactions with the cellular environment remain elusive. Here, we apply in situ cryo-correlative light and electron tomography to follow NC assembly and changes in VF morphology and their liquid properties during Ebola virus infection. We show that viral NCs transition from loosely packed helical assemblies in early VFs to compact cylinders that arrange into highly organized parallel bundles later in infection. Early VFs associate with intermediate filaments and are devoid of other host material but become progressively accessible to cellular components. Our data suggest that this process is coupled to VF solidification, loss of sphericity, and dispersion and promotes cytoplasmic exposure of NCs to facilitate their transport to budding sites. |
 External links | Cell / PubMed:39742805 |
| Methods | EM (tomography) / EM (subtomogram averaging) |
| Resolution | 21.0 Å |
| Structure data |  EMDB-18675: Cryo-ET of cryo-FIB milled Ebola virus infected Huh7 cells at 22 hours post infection.  EMDB-18676: Cryo-ET of cryo-FIB milled Ebola virus infected Huh7 cells at 22 hours post infection  EMDB-18678: Cryo-ET of cryo-FIB milled Ebola virus infected Huh7 cells at 14 hours post infection  EMDB-18679: Cryo-ET of cryo-FIB milled of Huh7 cells transfected with Ebola virus proteins NP, VP35, L, VP30-GFP vitrified at 28 hours post transfection  EMDB-18686: Cryo-ET of cryo-FIB milled of Huh7 cells transfected with Ebola virus proteins NP, VP35, L, VP30-GFP, VP40 and VP24 vitrified at 28 hours post transfection  EMDB-18690: Cryo-ET of cryo-FIB milled Ebola virus-VP30-GFP infected Huh7 cells at 14 hours post infection  EMDB-18695: Cryo-ET of cryo-FIB milled Ebola virus-VP30-GFP infected Huh7 cells at 22 hours post infection  EMDB-18696: Cryo-ET of cryo-FIB milled Ebola virus infected Huh7 cells at 14 hours post infection  EMDB-18697: Subtomogram average of Ebola virus nucleocapsid obtained from cryo-FIB milled Ebola virus infected Huh7 cells at 22 hours post infection |
