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TitleStructure of the native γ-tubulin ring complex capping spindle microtubules.
Journal, issue, pagesNat Struct Mol Biol, Vol. 31, Issue 7, Page 1134-1144, Year 2024
Publish dateApr 12, 2024
AuthorsTom Dendooven / Stanislau Yatskevich / Alister Burt / Zhuo A Chen / Dom Bellini / Juri Rappsilber / John V Kilmartin / David Barford /
PubMed AbstractMicrotubule (MT) filaments, composed of α/β-tubulin dimers, are fundamental to cellular architecture, function and organismal development. They are nucleated from MT organizing centers by the ...Microtubule (MT) filaments, composed of α/β-tubulin dimers, are fundamental to cellular architecture, function and organismal development. They are nucleated from MT organizing centers by the evolutionarily conserved γ-tubulin ring complex (γTuRC). However, the molecular mechanism of nucleation remains elusive. Here we used cryo-electron tomography to determine the structure of the native γTuRC capping the minus end of a MT in the context of enriched budding yeast spindles. In our structure, γTuRC presents a ring of γ-tubulin subunits to seed nucleation of exclusively 13-protofilament MTs, adopting an active closed conformation to function as a perfect geometric template for MT nucleation. Our cryo-electron tomography reconstruction revealed that a coiled-coil protein staples the first row of α/β-tubulin of the MT to alternating positions along the γ-tubulin ring of γTuRC. This positioning of α/β-tubulin onto γTuRC suggests a role for the coiled-coil protein in augmenting γTuRC-mediated MT nucleation. Based on our results, we describe a molecular model for budding yeast γTuRC activation and MT nucleation.
External linksNat Struct Mol Biol / PubMed:38609662 / PubMed Central
MethodsEM (subtomogram averaging) / X-ray diffraction
Resolution1.87 - 9.2 Å
Structure data

18664

8qv0

EMDB-18664, PDB-8qv0:
Structure of the native microtubule lattice nucleated from the yeast spindle pole body
Method: EM (subtomogram averaging) / Resolution: 6.6 Å

18665

8qv2

EMDB-18665, PDB-8qv2:
Structure of the native y-Tubulin Ring Complex (yTuRC) capping microtubule minus ends at the spindle pole body
Method: EM (subtomogram averaging) / Resolution: 9.2 Å

18666

8qv3

EMDB-18666, PDB-8qv3:
Structure of the y-Tubulin Small Complex (yTuSC) as part of the native y-Tubulin Ring Complex (yTuRC) capping microtubule minus ends at the spindle pole body
Method: EM (subtomogram averaging) / Resolution: 8.2 Å

PDB-8qry:
Crystal structure of the yeast spindle body component Spc98
Method: X-RAY DIFFRACTION / Resolution: 1.87 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsCELL CYCLE / yeast cell division / yeast spindle pole body / Microtubule nucleation / MTOC / y-tubulin / SPB

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