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- EMDB-18666: Structure of the y-Tubulin Small Complex (yTuSC) as part of the n... -

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Basic information

Entry
Database: EMDB / ID: EMD-18666
TitleStructure of the y-Tubulin Small Complex (yTuSC) as part of the native y-Tubulin Ring Complex (yTuRC) capping microtubule minus ends at the spindle pole body
Map data
Sample
  • Organelle or cellular component: y-Tubulin Small Complex bound to two Alpha/Beta tubulin dimers
    • Protein or peptide: Tubulin gamma chain
    • Protein or peptide: Spindle pole body component
    • Protein or peptide: Spindle pole body component
    • Protein or peptide: Spindle pole body component 110
    • Protein or peptide: Tubulin alpha-1 chain
    • Protein or peptide: Tubulin beta chain
    • Protein or peptide: Unknown protein
    • Protein or peptide: Unknown protein
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: water
KeywordsMicrotubule nucleation / MTOC / y-tubulin / SPB / CELL CYCLE
Function / homology
Function and homology information


equatorial microtubule organizing center / nuclear migration by microtubule mediated pushing forces / microtubule minus-end binding / nuclear division / mitotic spindle pole body / Platelet degranulation / homologous chromosome segregation / nuclear migration along microtubule / gamma-tubulin complex / meiotic spindle organization ...equatorial microtubule organizing center / nuclear migration by microtubule mediated pushing forces / microtubule minus-end binding / nuclear division / mitotic spindle pole body / Platelet degranulation / homologous chromosome segregation / nuclear migration along microtubule / gamma-tubulin complex / meiotic spindle organization / microtubule nucleation / gamma-tubulin binding / spindle pole body / tubulin complex / mitotic sister chromatid segregation / spindle assembly / cytoplasmic microtubule organization / nuclear periphery / mitotic spindle organization / meiotic cell cycle / structural constituent of cytoskeleton / microtubule cytoskeleton organization / spindle / spindle pole / mitotic cell cycle / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / hydrolase activity / GTPase activity / GTP binding / metal ion binding / nucleus / cytoplasm
Similarity search - Function
Spindle pole body component 110, C-terminal / Spindle pole body component 110 C-terminal domain / Gamma tubulin / Gamma tubulin complex component, C-terminal / Gamma-tubulin complex component, C-terminal domain superfamily / Gamma tubulin complex component C-terminal / Gamma-tubulin complex component protein / Gamma tubulin complex component protein, N-terminal / Gamma tubulin complex component N-terminal / Alpha tubulin ...Spindle pole body component 110, C-terminal / Spindle pole body component 110 C-terminal domain / Gamma tubulin / Gamma tubulin complex component, C-terminal / Gamma-tubulin complex component, C-terminal domain superfamily / Gamma tubulin complex component C-terminal / Gamma-tubulin complex component protein / Gamma tubulin complex component protein, N-terminal / Gamma tubulin complex component N-terminal / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily
Similarity search - Domain/homology
Tubulin beta chain / SPC98 isoform 1 / Tubulin gamma chain / Spindle pole body component / Spindle pole body component 110 / Tubulin alpha-1 chain
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
Methodsubtomogram averaging / cryo EM / Resolution: 8.2 Å
AuthorsDendooven T / Yatskevich S / Burt A / Bellini D / Kilmartin J / Barford D
Funding support United Kingdom, Germany, 3 items
OrganizationGrant numberCountry
Cancer Research UK United Kingdom
Boehringer Ingelheim Fonds (BIF) Germany
Medical Research Council (MRC, United Kingdom) United Kingdom
CitationJournal: Nat Struct Mol Biol / Year: 2024
Title: Structure of the native γ-tubulin ring complex capping spindle microtubules.
Authors: Tom Dendooven / Stanislau Yatskevich / Alister Burt / Zhuo A Chen / Dom Bellini / Juri Rappsilber / John V Kilmartin / David Barford /
Abstract: Microtubule (MT) filaments, composed of α/β-tubulin dimers, are fundamental to cellular architecture, function and organismal development. They are nucleated from MT organizing centers by the ...Microtubule (MT) filaments, composed of α/β-tubulin dimers, are fundamental to cellular architecture, function and organismal development. They are nucleated from MT organizing centers by the evolutionarily conserved γ-tubulin ring complex (γTuRC). However, the molecular mechanism of nucleation remains elusive. Here we used cryo-electron tomography to determine the structure of the native γTuRC capping the minus end of a MT in the context of enriched budding yeast spindles. In our structure, γTuRC presents a ring of γ-tubulin subunits to seed nucleation of exclusively 13-protofilament MTs, adopting an active closed conformation to function as a perfect geometric template for MT nucleation. Our cryo-electron tomography reconstruction revealed that a coiled-coil protein staples the first row of α/β-tubulin of the MT to alternating positions along the γ-tubulin ring of γTuRC. This positioning of α/β-tubulin onto γTuRC suggests a role for the coiled-coil protein in augmenting γTuRC-mediated MT nucleation. Based on our results, we describe a molecular model for budding yeast γTuRC activation and MT nucleation.
History
DepositionOct 17, 2023-
Header (metadata) releaseApr 24, 2024-
Map releaseApr 24, 2024-
UpdateJul 31, 2024-
Current statusJul 31, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_18666.png

Downloads & links

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Map

FileDownload / File: emd_18666.map.gz / Format: CCP4 / Size: 18.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
3.3 Å/pix.
x 168 pix.
= 554.4 Å		3.3 Å/pix.
x 168 pix.
= 554.4 Å		3.3 Å/pix.
x 168 pix.
= 554.4 Å

Surface

Projections

Slices (1/3)

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Images are generated by Spider.

Voxel sizeX=Y=Z: 3.3 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.163
Minimum - Maximum-0.3074678 - 1.074934
Average (Standard dev.)0.0032631468 (±0.037740443)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions168168168
Spacing168168168
CellA=B=C: 554.39996 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Map filtered with OccuPy

Fileemd_18666_additional_1.map
AnnotationMap filtered with OccuPy
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_18666_half_map_1.map
Projections & Slices
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Histogram

Histogram (log scale)

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Half map: #1

Fileemd_18666_half_map_2.map
Projections & Slices
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Sample components

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Entire : y-Tubulin Small Complex bound to two Alpha/Beta tubulin dimers

EntireName: y-Tubulin Small Complex bound to two Alpha/Beta tubulin dimers
Components
  • Organelle or cellular component: y-Tubulin Small Complex bound to two Alpha/Beta tubulin dimers
    • Protein or peptide: Tubulin gamma chain
    • Protein or peptide: Spindle pole body component
    • Protein or peptide: Spindle pole body component
    • Protein or peptide: Spindle pole body component 110
    • Protein or peptide: Tubulin alpha-1 chain
    • Protein or peptide: Tubulin beta chain
    • Protein or peptide: Unknown protein
    • Protein or peptide: Unknown protein
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: water

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Supramolecule #1: y-Tubulin Small Complex bound to two Alpha/Beta tubulin dimers

SupramoleculeName: y-Tubulin Small Complex bound to two Alpha/Beta tubulin dimers
type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1-#8
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Macromolecule #1: Tubulin gamma chain

MacromoleculeName: Tubulin gamma chain / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 52.671188 KDa
SequenceString: MGGEIITLQA GQCGNHVGKF LWSQLAKEHA IGTDGLSQLP DSSTERDDDT KPFFRENSRN KFTPRAIMMD SEPSVIADVE NTFRGFFDP RNTWVASDGA SAGNSWANGY DIGTRNQDDI LNKIDKEIDS TDNFEGFQLL HSVAGGTGSG LGSNLLEALC D RYPKKILT ...String:
MGGEIITLQA GQCGNHVGKF LWSQLAKEHA IGTDGLSQLP DSSTERDDDT KPFFRENSRN KFTPRAIMMD SEPSVIADVE NTFRGFFDP RNTWVASDGA SAGNSWANGY DIGTRNQDDI LNKIDKEIDS TDNFEGFQLL HSVAGGTGSG LGSNLLEALC D RYPKKILT TYSVFPARSS EVVVQSYNTI LALRRLIEDS DATVVFDNAS LLNISGKVFR NPNIDLQHTN QLISTIISSV TN SIRFPSY MYSSMSSIYS TLIPSPELHF LSPSFTPFTS DYIHDDIAHK GHSSYDVMLD LLDPSNSLVS TAMNNPTYFN VYN TIIGNV EPRQISRAMT KLQQRIKFPS WSSSAMHVNI GRRSPYLPLQ PNENEVSGMM LSNMSTVVNV FENACNTFDK VFAK GAFLN NYNVGDLFQS MQNVQDEFAE SREVVQSLME DYVAAEQDSY LDDVLVDDEN MVGELEEDLD ADGDHKLV

UniProtKB: Tubulin gamma chain

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Macromolecule #2: Spindle pole body component

MacromoleculeName: Spindle pole body component / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 96.940594 KDa
SequenceString: MEIKEVDDRA ELLRYTNNIP LLGKLVNHQP LWSTNPKLKS FSLEKISAPD QRRVQEALVV KDLLNVLIGL EGTYIRYFND YEPSDPETP IEFKIAKKMD PSFKTFSRRI VRYGKQYMIL TRAYEKWSDT SFGMVLQRFA YEIRRFLEDV YLKTLVERLE R DFNKVPNF ...String:
MEIKEVDDRA ELLRYTNNIP LLGKLVNHQP LWSTNPKLKS FSLEKISAPD QRRVQEALVV KDLLNVLIGL EGTYIRYFND YEPSDPETP IEFKIAKKMD PSFKTFSRRI VRYGKQYMIL TRAYEKWSDT SFGMVLQRFA YEIRRFLEDV YLKTLVERLE R DFNKVPNF SIRELEQIIN ETEVNKQMEL LYNIYEEIFR EIEERRTNQS SQEDFNNFMD SMKNESSLHL RLMVAFDTTV YP VPKGGAI LKIFQQKILE NLGDRSSVMF LKKLLNNISQ DYCTMLYEWL TQGILNDPYQ EFMTYDDLEG KTDNIFDTRD RAW DTQYFI RKDVLLRDCD SEEDKNLLFK MLRTGILLKV VRASLQIPTI PSNSSDITIQ EINDFADLME GSNLELYVDK CYSR ANEIF LKLFFQGYDL INVLKHLQQI FLGYQSGHNV LKFLTKNMGE LTKHYRNDNN ANYDKLLQNF ELERQSENPN NLMRQ LLMI QFDTETLPQV LSHYLQIYPE VPENNSANDD SDPLMHANNF KNMNAILFDE LSKERTGAYH GSNLELYTPK SAIYHL KFD INIPYPLNII ISRTCMIKYQ IILRYQLVLQ YHSRLLDETW MDLNKTPSWK YRGYSHTVKR RIVRATRVLH AKMNHFI KT IMEYFNQNVI DKEVYSLEKC YRNPTLAVAI QNELEGGLTN IMTNRCLSDL IPLQLQIFDI VYKFCKFIKS MRAKLCQL D PVLYEKHKSG MMKTLNEGYR TNNGGQEDVG YQEDAALELI QKLIEYISNA SSIFRKCLIN FTQELSTEKF DFYDSSSVD AAGIERVLYS IVPPRSASAS SQR

UniProtKB: Spindle pole body component

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Macromolecule #3: Spindle pole body component

MacromoleculeName: Spindle pole body component / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 98.336211 KDa
SequenceString: MELEPTLFGI IEALAPQLLS QSHLQTFVSD VVNLLRSSTK SATQLGPLID FYKLQSLDSP ETTIMWHKIE KFLDALFGIQ NTDDMVKYL SVFQSLLPSN YRAKIVQKSS GLNMENLANH EHLLSPVRAP SIYTEASFEN MDRFSERRSM VSSPNRYVPS S TYSSVTLR ...String:
MELEPTLFGI IEALAPQLLS QSHLQTFVSD VVNLLRSSTK SATQLGPLID FYKLQSLDSP ETTIMWHKIE KFLDALFGIQ NTDDMVKYL SVFQSLLPSN YRAKIVQKSS GLNMENLANH EHLLSPVRAP SIYTEASFEN MDRFSERRSM VSSPNRYVPS S TYSSVTLR QLSNPYYVNT IPEEDILKYV SYTLLATTSA LFPFDHEQIQ IPSKIPNFES GLLHLIFEAG LLYQSLGYKV EK FRMLNIS PMKKALIIEI SEELQNYTAF VNNLVSSGTV VSLKSLYREI YENIIRLRIY CRFTEHLEEL SGDTFLIELN IFK SHGDLT IRKIATNLFN SMISLYYEYL MNWLTKGLLR ATYGEFFIAE NTDTNGTDDD FIYHIPIEFN QERVPAFIPK ELAY KIFMI GKSYIFLEKY CKEVQWTNEF SKKYHVLYQS NSYRGISTNF FEIINDQYSE IVNHTNQILN QKFHYRDVVF ALKNI LLMG KSDFMDALIE KANDILATPS DSLPNYKLTR VLQEAVQLSS LRHLMNSPRN SSVINGLDAR VLDLGHGSVG WDVFTL DYI LYPPLSLVLN VNRPFGRKEY LRIFNFLWRF KKNNYFYQKE MLKSNDIIRS FKKIRGYNPL IRDIINKLSR ISILRTQ FQ QFNSKMESYY LNCIIEENFK EMTRKLQRTE NKSQNQFDLI RLNNGTIELN GILTPKAEVL TKSSSSKPQK HAIEKTLN I DELESVHNTF LTNILSHKLF ATNTSEISVG DYSGQPYPTS LVLLLNSVYE FVKVYCNLND IGYEIFIKMN LNDHEASNG LLGKFNTNLK EIVSQYKNFK DRLYIFRADL KNDGDEELFL LSKSLR

UniProtKB: SPC98 isoform 1

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Macromolecule #4: Spindle pole body component 110

MacromoleculeName: Spindle pole body component 110 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 111.987125 KDa
SequenceString: MDEASHLPNG SLKNMEFTPV GFIKSKRNTT QTQVVSPTKV PNANNGDENE GPVKKRQRRS IDDTIDSTRL FSEASQFDDS FPEIKANIP PSPRSGNVDK SRKRNLIDDL KKDVPMSQPL KEQEVREHQM KKERFDRALE SKLLGKRHIT YANSDISNKE L YINEIKSL ...String:
MDEASHLPNG SLKNMEFTPV GFIKSKRNTT QTQVVSPTKV PNANNGDENE GPVKKRQRRS IDDTIDSTRL FSEASQFDDS FPEIKANIP PSPRSGNVDK SRKRNLIDDL KKDVPMSQPL KEQEVREHQM KKERFDRALE SKLLGKRHIT YANSDISNKE L YINEIKSL KHEIKELRKE KNDTLNNYDT LEEETDDLKN RLQALEKELD AKNKIVNSRK VDDHSGCIEE REQMERKLAE LE RKLKTVK DQVLELENNS DVQSLKLRSK EDELKNLMNE LNELKSNAEE KDTQLEFKKN ELRKRTNELN ELKIKSDEMD LQL KQKQNE SKRLKDELNE LETKFSENGS QSSAKENELK MLKNKIAELE EEISTKNSQL IAKEGKLASL MAQLTQLESK LNQR DSQLG SREEELKKTN DKLQKDIRIA REETVSKDER IIDLQKKVKQ LENDLFVIKK THSESKTITD NELESKDKLI KILEN DLKV AQEKYSKMEK ELKEREFNYK ISESKLEDEK TTLNEKISNL AAENSQLKNK IEDNSTATHH MKENYEKQLE SLRKDI EEY KESAKDSEDK IEELKIRIAE NSAKVSEKRS KDIKQKDEQI SDLTQNLKLQ EDEISSLKSI IDRYKKDFNQ LKSEQSN IQ HDLNLQILNL ENKLIESEDE LKSLRDSQKI EIENWKRKYN NLSLENDRLL TEKESASDKE REISILNRKL DEMDKEKW N LQESKEKYKR ELQKVITAND RLRREKEELN ENSNNIRIME DKMTRIKKNY LSEITSLQEE NRRLEERLIL NERRKDNDS TMQLNDIISY YKLKYHSEVR HNNDLKVIND YLNKVLALGT RRLRLDTRKG EHSLNISLPD DDELDRDYYN SHVYTRYHDY EYPLRFNLN RRGPYFERRL SFKTVALLVL ACVRMKRIAF YRRSDDNRLR ILRDRIESSS GRISW

UniProtKB: Spindle pole body component 110

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Macromolecule #5: Tubulin alpha-1 chain

MacromoleculeName: Tubulin alpha-1 chain / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 49.853867 KDa
SequenceString: MREVISINVG QAGCQIGNAC WELYSLEHGI KPDGHLEDGL SKPKGGEEGF STFFHETGYG KFVPRAIYVD LEPNVIDEVR NGPYKDLFH PEQLISGKED AANNYARGHY TVGREILGDV LDRIRKLADQ CDGLQGFLFT HSLGGGTGSG LGSLLLEELS A EYGKKSKL ...String:
MREVISINVG QAGCQIGNAC WELYSLEHGI KPDGHLEDGL SKPKGGEEGF STFFHETGYG KFVPRAIYVD LEPNVIDEVR NGPYKDLFH PEQLISGKED AANNYARGHY TVGREILGDV LDRIRKLADQ CDGLQGFLFT HSLGGGTGSG LGSLLLEELS A EYGKKSKL EFAVYPAPQV STSVVEPYNT VLTTHTTLEH ADCTFMVDNE AIYDMCKRNL DIPRPSFANL NNLIAQVVSS VT ASLRFDG SLNVDLNEFQ TNLVPYPRIH FPLVSYSPVL SKSKAFHESN SVSEITNACF EPGNQMVKCD PRDGKYMATC LLY RGDVVT RDVQRAVEQV KNKKTVQLVD WCPTGFKIGI CYEPPTATPN SQLATVDRAV CMLSNTTSIA EAWKRIDRKF DLMY AKRAF VHWYVGEGME EGEFTEARED LAALERDYIE VGADSYAEEE EF

UniProtKB: Tubulin alpha-1 chain

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Macromolecule #6: Tubulin beta chain

MacromoleculeName: Tubulin beta chain / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 50.967457 KDa
SequenceString: MREIIHISTG QCGNQIGAAF WETICGEHGL DFNGTYHGHD DIQKERLNVY FNEASSGKWV PRSINVDLEP GTIDAVRNSA IGNLFRPDN YIFGQSSAGN VWAKGHYTEG AELVDSVMDV IRREAEGCDS LQGFQITHSL GGGTGSGMGT LLISKIREEF P DRMMATFS ...String:
MREIIHISTG QCGNQIGAAF WETICGEHGL DFNGTYHGHD DIQKERLNVY FNEASSGKWV PRSINVDLEP GTIDAVRNSA IGNLFRPDN YIFGQSSAGN VWAKGHYTEG AELVDSVMDV IRREAEGCDS LQGFQITHSL GGGTGSGMGT LLISKIREEF P DRMMATFS VLPSPKTSDT VVEPYNATLS VHQLVEHSDE TFCIDNEALY DICQRTLKLN QPSYGDLNNL VSSVMSGVTT SL RYPGQLN SDLRKLAVNL VPFPRLHFFM VGYAPLTAIG SQSFRSLTVP ELTQQMFDAK NMMAAADPRN GRYLTVAAFF RGK VSVKEV EDEMHKVQSK NSDYFVEWIP NNVQTAVCSV APQGLDMAAT FIANSTSIQE LFKRVGDQFS AMFKRKAFLH WYTS EGMDE LEFSEAESNM NDLVSEYQQY QEATVEDDEE VDENGDFGAP QNQDEPITEN FE

UniProtKB: Tubulin beta chain

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Macromolecule #7: Unknown protein

MacromoleculeName: Unknown protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 5.720042 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)

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Macromolecule #8: Unknown protein

MacromoleculeName: Unknown protein / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 5.294518 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #9: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 9 / Number of copies: 2 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

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Macromolecule #10: water

MacromoleculeName: water / type: ligand / ID: 10 / Number of copies: 1 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation stateparticle

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Sample preparation

BufferpH: 6.53
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 3.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.5 µm / Nominal defocus min: 2.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 8.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 31720
ExtractionNumber tomograms: 364 / Number images used: 31720
Final angle assignmentType: MAXIMUM LIKELIHOOD

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Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

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