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TitleMechanism of mammalian transcriptional repression by noncoding RNA.
Journal, issue, pagesNat Struct Mol Biol, Vol. 32, Issue 4, Page 607-612, Year 2025
Publish dateJan 6, 2025
AuthorsKatarína Tlučková / Beata Kaczmarek / Anita Salmazo / Carrie Bernecky /
PubMed AbstractTranscription by RNA polymerase II (Pol II) can be repressed by noncoding RNA, including the human RNA Alu. However, the mechanism by which endogenous RNAs repress transcription remains unclear. Here ...Transcription by RNA polymerase II (Pol II) can be repressed by noncoding RNA, including the human RNA Alu. However, the mechanism by which endogenous RNAs repress transcription remains unclear. Here we present cryogenic-electron microscopy structures of Pol II bound to Alu RNA, which reveal that Alu RNA mimics how DNA and RNA bind to Pol II during transcription elongation. Further, we show how distinct domains of the general transcription factor TFIIF control repressive activity. Together, we reveal how a noncoding RNA can regulate mammalian gene expression.
External linksNat Struct Mol Biol / PubMed:39762629 / PubMed Central
MethodsEM (single particle)
Resolution2.4 - 3.2 Å
Structure data

EMDB-18367: RNA polymerase II bound to Alu RNA left arm
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-18371: RNA polymerase II bound to minimal Alu RNA right arm in the canonical conformation
Method: EM (single particle) / Resolution: 3.2 Å

18375

8qep

EMDB-18375, PDB-8qep:
RNA polymerase II bound to Alu RNA right arm
Method: EM (single particle) / Resolution: 2.5 Å

18376

8qeq

EMDB-18376, PDB-8qeq:
RNA polymerase II bound to minimal Alu RNA right arm in the alternative conformation
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • sus scrofa domesticus (domestic pig)
  • homo sapiens (human)
KeywordsTRANSCRIPTION / transcription inhibition / noncoding RNA / RNA polymerase II / Alu RNA

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