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TitleEngineering the ADDobody protein scaffold for generation of high-avidity ADDomer super-binders.
Journal, issue, pagesStructure, Vol. 32, Issue 3, Page 342-351.e6, Year 2024
Publish dateMar 7, 2024
AuthorsDora Buzas / Huan Sun / Christine Toelzer / Sathish K N Yadav / Ufuk Borucu / Gunjan Gautam / Kapil Gupta / Joshua C Bufton / Julien Capin / Richard B Sessions / Frederic Garzoni / Imre Berger / Christiane Schaffitzel /
PubMed AbstractAdenovirus-derived nanoparticles (ADDomer) comprise 60 copies of adenovirus penton base protein (PBP). ADDomer is thermostable, rendering the storage, transport, and deployment of ADDomer-based ...Adenovirus-derived nanoparticles (ADDomer) comprise 60 copies of adenovirus penton base protein (PBP). ADDomer is thermostable, rendering the storage, transport, and deployment of ADDomer-based therapeutics independent of a cold chain. To expand the scope of ADDomers for new applications, we engineered ADDobodies, representing PBP crown domain, genetically separated from PBP multimerization domain. We inserted heterologous sequences into hyper-variable loops, resulting in monomeric, thermostable ADDobodies expressed at high yields in Escherichia coli. The X-ray structure of an ADDobody prototype validated our design. ADDobodies can be used in ribosome display experiments to select a specific binder against a target, with an enrichment factor of ∼10-fold per round. ADDobodies can be re-converted into ADDomers by genetically reconnecting the selected ADDobody with the PBP multimerization domain from a different species, giving rise to a multivalent nanoparticle, called Chimera, confirmed by a 2.2 Å electron cryo-microscopy structure. Chimera comprises 60 binding sites, resulting in ultra-high, picomolar avidity to the target.
External linksStructure / PubMed:38198950
MethodsEM (single particle) / X-ray diffraction
Resolution2.2 - 3.17 Å
Structure data

18323

8qbx

EMDB-18323, PDB-8qbx:
Chimeric Adenovirus-derived dodecamer
Method: EM (single particle) / Resolution: 2.2 Å

PDB-8coi:
Human adenovirus-derived synthetic ADDobody binder
Method: X-RAY DIFFRACTION / Resolution: 3.17 Å

PDB-8qb3:
ADDobody zinc containing condition
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • human adenovirus sp.
KeywordsVIRAL PROTEIN / penton base / crown domain / human adenovirus / Adenovirus capsid / Penton base protein / Structural viral protein / VIRUS LIKE PARTICLE / Adenovirus / capsid protein / virus-like particle

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