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TitleCryo-EM structure of influenza helical nucleocapsid reveals NP-NP and NP-RNA interactions as a model for the genome encapsidation.
Journal, issue, pagesSci Adv, Vol. 9, Issue 50, Page eadj9974, Year 2023
Publish dateDec 15, 2023
AuthorsFlorian Chenavier / Leandro F Estrozi / Jean-Marie Teulon / Eleftherios Zarkadas / Lily-Lorette Freslon / Jean-Luc Pellequer / Rob W H Ruigrok / Guy Schoehn / Allison Ballandras-Colas / Thibaut Crépin /
PubMed AbstractInfluenza virus genome encapsidation is essential for the formation of a helical viral ribonucleoprotein (vRNP) complex composed of nucleoproteins (NP), the trimeric polymerase, and the viral genome. ...Influenza virus genome encapsidation is essential for the formation of a helical viral ribonucleoprotein (vRNP) complex composed of nucleoproteins (NP), the trimeric polymerase, and the viral genome. Although low-resolution vRNP structures are available, it remains unclear how the viral RNA is encapsidated and how NPs assemble into the helical filament specific of influenza vRNPs. In this study, we established a biological tool, the RNP-like particles assembled from recombinant influenza A virus NP and synthetic RNA, and we present the first subnanometric cryo-electron microscopy structure of the helical NP-RNA complex (8.7 to 5.3 Å). The helical RNP-like structure reveals a parallel double-stranded conformation, allowing the visualization of NP-NP and NP-RNA interactions. The RNA, located at the interface of neighboring NP protomers, interacts with conserved residues previously described as essential for the NP-RNA interaction. The NP undergoes conformational changes to enable RNA binding and helix formation. Together, our findings provide relevant insights for understanding the mechanism for influenza genome encapsidation.
External linksSci Adv / PubMed:38100595 / PubMed Central
MethodsEM (helical sym.)
Resolution5.3 - 8.7 Å
Structure data

18043

8pzp

EMDB-18043: Helical structure of the influenza A virus ribonucleoprotein-like
PDB-8pzp: Model for influenza A virus helical ribonucleoprotein-like structure
Method: EM (helical sym.) / Resolution: 8.7 Å

18044

8pzq

EMDB-18044: Focused reconstruction of influenza A RNP-like particle
PDB-8pzq: Model for focused reconstruction of influenza A RNP-like particle
Method: EM (helical sym.) / Resolution: 5.3 Å

Source
  • influenza a virus (a/wsn/1933(h1n1))
  • synthetic construct (others)
  • influenza a virus
KeywordsVIRAL PROTEIN / Influenza virus / Nucleocapsid-like / RNA binding protein.

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