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-Structure paper
Title | Long-range charge transfer mechanism of the IIIIV mycobacterial supercomplex. |
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Journal, issue, pages | Nat Commun, Vol. 15, Issue 1, Page 5276, Year 2024 |
Publish date | Jun 20, 2024 |
![]() | Daniel Riepl / Ana P Gamiz-Hernandez / Terezia Kovalova / Sylwia M Król / Sophie L Mader / Dan Sjöstrand / Martin Högbom / Peter Brzezinski / Ville R I Kaila / ![]() |
PubMed Abstract | Aerobic life is powered by membrane-bound redox enzymes that shuttle electrons to oxygen and transfer protons across a biological membrane. Structural studies suggest that these energy-transducing ...Aerobic life is powered by membrane-bound redox enzymes that shuttle electrons to oxygen and transfer protons across a biological membrane. Structural studies suggest that these energy-transducing enzymes operate as higher-order supercomplexes, but their functional role remains poorly understood and highly debated. Here we resolve the functional dynamics of the 0.7 MDa IIIIV obligate supercomplex from Mycobacterium smegmatis, a close relative of M. tuberculosis, the causative agent of tuberculosis. By combining computational, biochemical, and high-resolution (2.3 Å) cryo-electron microscopy experiments, we show how the mycobacterial supercomplex catalyses long-range charge transport from its menaquinol oxidation site to the binuclear active site for oxygen reduction. Our data reveal proton and electron pathways responsible for the charge transfer reactions, mechanistic principles of the quinone catalysis, and how unique molecular adaptations, water molecules, and lipid interactions enable the proton-coupled electron transfer (PCET) reactions. Our combined findings provide a mechanistic blueprint of mycobacterial supercomplexes and a basis for developing drugs against pathogenic bacteria. |
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Methods | EM (single particle) |
Resolution | 2.3 - 2.8 Å |
Structure data | EMDB-17210, PDB-8ovc: EMDB-17211, PDB-8ovd: |
Chemicals | ![]() ChemComp-HEC: ![]() ChemComp-MQ9: ![]()
ChemComp-WUO: ![]() ChemComp-FES: ![]() ChemComp-9YF: ![]() ChemComp-HEM: ![]() ChemComp-CDL: ![]() ChemComp-7PH: ![]() ChemComp-HEA: ![]() ChemComp-CU: ![]() ChemComp-MG: ![]() ChemComp-CA: ![]() ChemComp-9XX: ![]() ChemComp-PLM: ![]() ChemComp-HOH: ![]() ChemComp-IZL: ![]() ChemComp-TRD: ![]() ChemComp-3PE: |
Source |
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![]() | ELECTRON TRANSPORT / RESPIRATORY SUPERCOMPLEX / MEMBRANE PROTEIN / ACTINOBACTERIA |