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TitleStructural and mechanistic insights into the CAND1-mediated SCF substrate receptor exchange.
Journal, issue, pagesMol Cell, Vol. 83, Issue 13, Page 2332-22346.e8, Year 2023
Publish dateJul 6, 2023
AuthorsMohammed Shaaban / Julie A Clapperton / Shan Ding / Simone Kunzelmann / Märt-Erik Mäeots / Sarah L Maslen / J Mark Skehel / Radoslav I Enchev /
PubMed AbstractModular SCF (SKP1-CUL1-Fbox) ubiquitin E3 ligases orchestrate multiple cellular pathways in eukaryotes. Their variable SKP1-Fbox substrate receptor (SR) modules enable regulated substrate recruitment ...Modular SCF (SKP1-CUL1-Fbox) ubiquitin E3 ligases orchestrate multiple cellular pathways in eukaryotes. Their variable SKP1-Fbox substrate receptor (SR) modules enable regulated substrate recruitment and subsequent proteasomal degradation. CAND proteins are essential for the efficient and timely exchange of SRs. To gain structural understanding of the underlying molecular mechanism, we reconstituted a human CAND1-driven exchange reaction of substrate-bound SCF alongside its co-E3 ligase DCNL1 and visualized it by cryo-EM. We describe high-resolution structural intermediates, including a ternary CAND1-SCF complex, as well as conformational and compositional intermediates representing SR- or CAND1-dissociation. We describe in molecular detail how CAND1-induced conformational changes in CUL1/RBX1 provide an optimized DCNL1-binding site and reveal an unexpected dual role for DCNL1 in CAND1-SCF dynamics. Moreover, a partially dissociated CAND1-SCF conformation accommodates cullin neddylation, leading to CAND1 displacement. Our structural findings, together with functional biochemical assays, help formulate a detailed model for CAND-SCF regulation.
External linksMol Cell / PubMed:37339624
MethodsEM (single particle)
Resolution2.9 - 8.3 Å
Structure data

17114

8or0

EMDB-17114, PDB-8or0:
CAND1-CUL1-RBX1-SKP1-SKP2-CKS1-CDK2
Method: EM (single particle) / Resolution: 3.1 Å

17115

8or2

EMDB-17115, PDB-8or2:
CAND1-CUL1-RBX1-DCNL1
Method: EM (single particle) / Resolution: 3.2 Å

17116

8or3

EMDB-17116, PDB-8or3:
CAND1-CUL1-RBX1-SKP1-SKP2-DCNL1
Method: EM (single particle) / Resolution: 2.9 Å

17117

8or4

EMDB-17117, PDB-8or4:
Partially dissociated CAND1-CUL1-RBX1-SKP1-SKP2-CKS1-CDK2
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-17124: CAND1-CUL1-RBX1
Method: EM (single particle) / Resolution: 8.3 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
KeywordsLIGASE / CAND1 / substrate receptor exchange factor / cullin-RING ligase / CRL / SCF / neddylation / DCNL1 co-E3 / ubiquitin signaling

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