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TitleAn original potentiating mechanism revealed by the cryo-EM structures of the human α7 nicotinic receptor in complex with nanobodies.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 5964, Year 2023
Publish dateSep 25, 2023
AuthorsMarie S Prevost / Nathalie Barilone / Gabrielle Dejean de la Bâtie / Stéphanie Pons / Gabriel Ayme / Patrick England / Marc Gielen / François Bontems / Gérard Pehau-Arnaudet / Uwe Maskos / Pierre Lafaye / Pierre-Jean Corringer /
PubMed AbstractThe human α7 nicotinic receptor is a pentameric channel mediating cellular and neuronal communication. It has attracted considerable interest in designing ligands for the treatment of neurological ...The human α7 nicotinic receptor is a pentameric channel mediating cellular and neuronal communication. It has attracted considerable interest in designing ligands for the treatment of neurological and psychiatric disorders. To develop a novel class of α7 ligands, we recently generated two nanobodies named E3 and C4, acting as positive allosteric modulator and silent allosteric ligand, respectively. Here, we solved the cryo-electron microscopy structures of the nanobody-receptor complexes. E3 and C4 bind to a common epitope involving two subunits at the apex of the receptor. They form by themselves a symmetric pentameric assembly that extends the extracellular domain. Unlike C4, the binding of E3 drives an agonist-bound conformation of the extracellular domain in the absence of an orthosteric agonist, and mutational analysis shows a key contribution of an N-linked sugar moiety in mediating E3 potentiation. The nanobody E3, by remotely controlling the global allosteric conformation of the receptor, implements an original mechanism of regulation that opens new avenues for drug design.
External linksNat Commun / PubMed:37749098 / PubMed Central
MethodsEM (single particle)
Resolution2.3 - 4.4 Å
Structure data

16513

8c9x

EMDB-16513, PDB-8c9x:
human alpha7 nicotinic receptor in complex with the C4 nanobody
Method: EM (single particle) / Resolution: 2.3 Å

16534

8cau

EMDB-16534, PDB-8cau:
human alpha7 nicotinic receptor in complex with the C4 nanobody and nicotine
Method: EM (single particle) / Resolution: 3.4 Å

16598

8ce4

EMDB-16598, PDB-8ce4:
Human alpha7 nicotinic receptor in complex with the E3 nanobody
Method: EM (single particle) / Resolution: 2.7 Å

16665

8ci1

EMDB-16665, PDB-8ci1:
Human alpha7 nicotinic receptor in complex with the E3 nanobody and nicotine
Method: EM (single particle) / Resolution: 2.8 Å

16666

8ci2

EMDB-16666, PDB-8ci2:
human alpha7 nicotinic receptor in complex with the C4 nanobody under sub-saturating conditions
Method: EM (single particle) / Resolution: 4.4 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-NCT:
(S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE / alkaloid*YM / Nicotine

Source
  • homo sapiens (human)
  • vicugna pacos huacaya (mammal)
  • vicugna pacos (alpaca)
KeywordsMEMBRANE PROTEIN / ion channel nanobody Nicotinic acetylcholine receptor

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