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| Title | Adenovirus core protein V reinforces the capsid and enhances genome release from disrupted particles. |
|---|---|
| Journal, issue, pages | Sci Adv, Vol. 9, Issue 14, Page eade9910, Year 2023 |
| Publish date | Apr 7, 2023 |
 Authors | Natalia Martín-González / Alfonso Gómez-González / Mercedes Hernando-Pérez / Michael Bauer / Urs F Greber / Carmen San Martín / Pedro J de Pablo /   |
| PubMed Abstract | Out of the three core proteins in human adenovirus, protein V is believed to connect the inner capsid surface to the outer genome layer. Here, we explored mechanical properties and in vitro ...Out of the three core proteins in human adenovirus, protein V is believed to connect the inner capsid surface to the outer genome layer. Here, we explored mechanical properties and in vitro disassembly of particles lacking protein V (Ad5-ΔV). Ad5-ΔV particles were softer and less brittle than the wild-type ones (Ad5-wt), but they were more prone to release pentons under mechanical fatigue. In Ad5-ΔV, core components did not readily diffuse out of partially disrupted capsids, and the core appeared more condensed than in Ad5-wt. These observations suggest that instead of condensing the genome, protein V antagonizes the condensing action of the other core proteins. Protein V provides mechanical reinforcement and facilitates genome release by keeping DNA connected to capsid fragments that detach during disruption. This scenario is in line with the location of protein V in the virion and its role in Ad5 cell entry. |
 External links | Sci Adv / PubMed:37027464 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 4.4 Å |
| Structure data |  EMDB-15694: Human adenovirus type 5 lacking core protein V |
