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-Structure paper
Title | An ancestral interaction module promotes oligomerization in divergent mitochondrial ATP synthases. |
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Journal, issue, pages | Nat Commun, Vol. 13, Issue 1, Page 5989, Year 2022 |
Publish date | Oct 11, 2022 |
Authors | Ondřej Gahura / Alexander Mühleip / Carolina Hierro-Yap / Brian Panicucci / Minal Jain / David Hollaus / Martina Slapničková / Alena Zíková / Alexey Amunts / |
PubMed Abstract | Mitochondrial ATP synthase forms stable dimers arranged into oligomeric assemblies that generate the inner-membrane curvature essential for efficient energy conversion. Here, we report cryo-EM ...Mitochondrial ATP synthase forms stable dimers arranged into oligomeric assemblies that generate the inner-membrane curvature essential for efficient energy conversion. Here, we report cryo-EM structures of the intact ATP synthase dimer from Trypanosoma brucei in ten different rotational states. The model consists of 25 subunits, including nine lineage-specific, as well as 36 lipids. The rotary mechanism is influenced by the divergent peripheral stalk, conferring a greater conformational flexibility. Proton transfer in the lumenal half-channel occurs via a chain of five ordered water molecules. The dimerization interface is formed by subunit-g that is critical for interactions but not for the catalytic activity. Although overall dimer architecture varies among eukaryotes, we find that subunit-g together with subunit-e form an ancestral oligomerization motif, which is shared between the trypanosomal and mammalian lineages. Therefore, our data defines the subunit-g/e module as a structural component determining ATP synthase oligomeric assemblies. |
External links | Nat Commun / PubMed:36220811 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.7 - 4.3 Å |
Structure data | EMDB-15559, PDB-8ap6: EMDB-15560, PDB-8ap7: EMDB-15561, PDB-8ap8: EMDB-15562, PDB-8ap9: EMDB-15563, PDB-8apa: EMDB-15564, PDB-8apb: EMDB-15565, PDB-8apc: EMDB-15566, PDB-8apd: EMDB-15567, PDB-8ape: EMDB-15568, PDB-8apf: EMDB-15570, PDB-8apg: EMDB-15571, PDB-8aph: EMDB-15572, PDB-8apj: EMDB-15573, PDB-8apk: |
Chemicals | ChemComp-ATP: ChemComp-MG: ChemComp-CDL: ChemComp-ADP: ChemComp-LMT: ChemComp-Q7G: ChemComp-PEE: ChemComp-PC1: ChemComp-UTP: ChemComp-HOH: ChemComp-3PE: |
Source |
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Keywords | MEMBRANE PROTEIN / ATP synthase / mitochondria |