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TitleAssembly mechanism and cryoEM structure of RecA recombination nucleofilaments from Streptococcus pneumoniae.
Journal, issue, pagesNucleic Acids Res, Vol. 51, Issue 6, Page 2800-2817, Year 2023
Publish dateApr 11, 2023
AuthorsMaud Hertzog / Thomas Noé Perry / Pauline Dupaigne / Sandra Serres / Violette Morales / Anne-Lise Soulet / Jason C Bell / Emmanuel Margeat / Stephen C Kowalczykowski / Eric Le Cam / Rémi Fronzes / Patrice Polard /
PubMed AbstractRecA-mediated homologous recombination (HR) is a key mechanism for genome maintenance and plasticity in bacteria. It proceeds through RecA assembly into a dynamic filament on ssDNA, the presynaptic ...RecA-mediated homologous recombination (HR) is a key mechanism for genome maintenance and plasticity in bacteria. It proceeds through RecA assembly into a dynamic filament on ssDNA, the presynaptic filament, which mediates DNA homology search and ordered DNA strand exchange. Here, we combined structural, single molecule and biochemical approaches to characterize the ATP-dependent assembly mechanism of the presynaptic filament of RecA from Streptococcus pneumoniae (SpRecA), in comparison to the Escherichia coli RecA (EcRecA) paradigm. EcRecA polymerization on ssDNA is assisted by the Single-Stranded DNA Binding (SSB) protein, which unwinds ssDNA secondary structures that block EcRecA nucleofilament growth. We report by direct microscopic analysis of SpRecA filamentation on ssDNA that neither of the two paralogous pneumococcal SSBs could assist the extension of SpRecA nucleopolymers. Instead, we found that the conserved RadA helicase promotes SpRecA nucleofilamentation in an ATP-dependent manner. This allowed us to solve the atomic structure of such a long native SpRecA nucleopolymer by cryoEM stabilized with ATPγS. It was found to be equivalent to the crystal structure of the EcRecA filament with a marked difference in how RecA mediates nucleotide orientation in the stretched ssDNA. Then, our results show that SpRecA and EcRecA HR activities are different, in correlation with their distinct ATP-dependent ssDNA binding modes.
External linksNucleic Acids Res / PubMed:36806960 / PubMed Central
MethodsEM (helical sym.)
Resolution3.8 - 3.9 Å
Structure data

15524

8amd

EMDB-15524, PDB-8amd:
Cryo-EM structure of the RecA presynaptic filament from S.pneumoniae
Method: EM (helical sym.) / Resolution: 3.9 Å

15525

8amf

EMDB-15525, PDB-8amf:
Cryo-EM structure of the RecA postsynaptic filament from S. pneumoniae
Method: EM (helical sym.) / Resolution: 3.8 Å

Chemicals

ChemComp-AGS:
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogue*YM

Source
  • streptococcus pneumoniae (bacteria)
  • bacteriophage sp. (virus)
  • lambdavirus lambda
KeywordsRECOMBINATION / Helical reconstruction / Recombinase / Streptococcus pneumoniae / single-stranded DNA / double-stranded DNA

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