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TitleYersinia entomophaga Tc toxin is released by T10SS-dependent lysis of specialized cell subpopulations.
Journal, issue, pagesNat Microbiol, Vol. 9, Issue 2, Page 390-404, Year 2024
Publish dateJan 18, 2024
AuthorsOleg Sitsel / Zhexin Wang / Petra Janning / Lara Kroczek / Thorsten Wagner / Stefan Raunser /
PubMed AbstractDisease-causing bacteria secrete numerous toxins to invade and subjugate their hosts. Unlike many smaller toxins, the secretion machinery of most large toxins remains enigmatic. By combining genomic ...Disease-causing bacteria secrete numerous toxins to invade and subjugate their hosts. Unlike many smaller toxins, the secretion machinery of most large toxins remains enigmatic. By combining genomic editing, proteomic profiling and cryo-electron tomography of the insect pathogen Yersinia entomophaga, we demonstrate that a specialized subset of these cells produces a complex toxin cocktail, including the nearly ribosome-sized Tc toxin YenTc, which is subsequently exported by controlled cell lysis using a transcriptionally coupled, pH-dependent type 10 secretion system (T10SS). Our results dissect the Tc toxin export process by a T10SS, identifying that T10SSs operate via a previously unknown lytic mode of action and establishing them as crucial players in the size-insensitive release of cytoplasmically folded toxins. With T10SSs directly embedded in Tc toxin operons of major pathogens, we anticipate that our findings may model an important aspect of pathogenesis in bacteria with substantial impact on agriculture and healthcare.
External linksNat Microbiol / PubMed:38238469 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution26.0 - 27.0 Å
Structure data

EMDB-15403: Structure of the secreted Yersinia entomophaga Tc toxin YenTc
Method: EM (subtomogram averaging) / Resolution: 26.0 Å

EMDB-15404: Tomogram of Yersinia entomophaga in the pre-secretory state
Method: EM (tomography)

EMDB-15405: Tomogram of Yersinia entomophaga in the post-holin state
Method: EM (tomography)

EMDB-15406: Tomogram of Yersinia entomophaga in the post-endolysin state
Method: EM (tomography)

EMDB-15407: Tomogram of Yersinia entomophaga in the post-spanin state
Method: EM (tomography)

EMDB-16538: Tomogram of Serratia marcescens in the post-spanin state (1)
Method: EM (tomography)

EMDB-16539: Tomogram of Serratia marcescens in the post-spanin state (2)
Method: EM (tomography)

EMDB-16618: Structure of Yersinia entomophaga Tc toxin from FIB-milled spheroplasts
Method: EM (subtomogram averaging) / Resolution: 27.0 Å

EMDB-16619: Tomogram of Yersinia entomophaga in the post-holin state (whole cell)
Method: EM (tomography)

Source
  • Yersinia entomophaga (bacteria)
  • Serratia marcescens (bacteria)

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