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TitleMechanism of RecF-RecO-RecR cooperation in bacterial homologous recombination.
Journal, issue, pagesNat Struct Mol Biol, Vol. 30, Issue 5, Page 650-660, Year 2023
Publish dateApr 20, 2023
AuthorsShivlee Nirwal / Mariusz Czarnocki-Cieciura / Anuradha Chaudhary / Weronika Zajko / Krzysztof Skowronek / Sebastian Chamera / Małgorzata Figiel / Marcin Nowotny /
PubMed AbstractIn bacteria, one type of homologous-recombination-based DNA-repair pathway involves RecFOR proteins that bind at the junction between single-stranded (ss) and double-stranded (ds) DNA. They ...In bacteria, one type of homologous-recombination-based DNA-repair pathway involves RecFOR proteins that bind at the junction between single-stranded (ss) and double-stranded (ds) DNA. They facilitate the replacement of SSB protein, which initially covers ssDNA, with RecA, which mediates the search for homologous sequences. However, the molecular mechanism of RecFOR cooperation remains largely unknown. We used Thermus thermophilus proteins to study this system. Here, we present a cryo-electron microscopy structure of the RecF-dsDNA complex, and another reconstruction that shows how RecF interacts with two different regions of the tetrameric RecR ring. Lower-resolution reconstructions of the RecR-RecO subcomplex and the RecFOR-DNA assembly explain how RecO is positioned to interact with ssDNA and SSB, which is proposed to lock the complex on a ssDNA-dsDNA junction. Our results integrate the biochemical data available for the RecFOR system and provide a framework for its complete understanding.
External linksNat Struct Mol Biol / PubMed:37081315
MethodsEM (single particle)
Resolution3.05 - 6.09 Å
Structure data

15231

8a8j

EMDB-15231, PDB-8a8j:
Complex of RecF and DNA from Thermus thermophilus.
Method: EM (single particle) / Resolution: 3.1 Å

15267

8a93

EMDB-15267, PDB-8a93:
Complex of RecF-RecR-DNA from Thermus thermophilus.
Method: EM (single particle) / Resolution: 3.05 Å

15308

8ab0

EMDB-15308, PDB-8ab0:
Complex of RecO-RecR-DNA from Thermus thermophilus.
Method: EM (single particle) / Resolution: 6.09 Å

16164

8bpr

EMDB-16164, PDB-8bpr:
Complex of RecF-RecO-RecR-DNA from Thermus thermophilus (low resolution reconstruction).
Method: EM (single particle) / Resolution: 3.65 Å

Chemicals

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • thermus thermophilus hb8 (bacteria)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN / DNA Repair pathway / RecFOR pathway / Thermus thermophilus

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