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TitleMechanism of RNA polymerase I selection by transcription factor UAF.
Journal, issue, pagesSci Adv, Vol. 8, Issue 16, Page eabn5725, Year 2022
Publish dateApr 22, 2022
AuthorsFlorence Baudin / Brice Murciano / Herman K H Fung / Simon A Fromm / Simone Mattei / Julia Mahamid / Christoph W Müller /
PubMed AbstractPreribosomal RNA is selectively transcribed by RNA polymerase (Pol) I in eukaryotes. The yeast transcription factor upstream activating factor (UAF) represses Pol II transcription and mediates Pol I ...Preribosomal RNA is selectively transcribed by RNA polymerase (Pol) I in eukaryotes. The yeast transcription factor upstream activating factor (UAF) represses Pol II transcription and mediates Pol I preinitiation complex (PIC) formation at the 35 ribosomal RNA gene. To visualize the molecular intermediates toward PIC formation, we determined the structure of UAF in complex with native promoter DNA and transcription factor TATA-box-binding protein (TBP). We found that UAF recognizes DNA using a hexameric histone-like scaffold with markedly different interactions compared with the nucleosome and the histone-fold-rich transcription factor IID (TFIID). In parallel, UAF positions TBP for Core Factor binding, which leads to Pol I recruitment, while sequestering it from DNA and Pol II/III-specific transcription factors. Our work thus reveals the structural basis of RNA Pol selection by a transcription factor.
External linksSci Adv / PubMed:35442737 / PubMed Central
MethodsEM (single particle)
Resolution2.8 Å
Structure data

14428

7z0o

EMDB-14428, PDB-7z0o:
Structure of transcription factor UAF in complex with TBP and 35S rRNA promoter DNA
Method: EM (single particle) / Resolution: 2.8 Å

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSCRIPTION / Transcription Factor / Nucleolus / Histone Fold

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