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-Structure paper
| タイトル | Mechanism of RNA polymerase I selection by transcription factor UAF. |
|---|---|
| ジャーナル・号・ページ | Sci Adv, Vol. 8, Issue 16, Page eabn5725, Year 2022 |
| 掲載日 | 2022年4月22日 |
著者 | Florence Baudin / Brice Murciano / Herman K H Fung / Simon A Fromm / Simone Mattei / Julia Mahamid / Christoph W Müller / ![]() |
| PubMed 要旨 | Preribosomal RNA is selectively transcribed by RNA polymerase (Pol) I in eukaryotes. The yeast transcription factor upstream activating factor (UAF) represses Pol II transcription and mediates Pol I ...Preribosomal RNA is selectively transcribed by RNA polymerase (Pol) I in eukaryotes. The yeast transcription factor upstream activating factor (UAF) represses Pol II transcription and mediates Pol I preinitiation complex (PIC) formation at the 35 ribosomal RNA gene. To visualize the molecular intermediates toward PIC formation, we determined the structure of UAF in complex with native promoter DNA and transcription factor TATA-box-binding protein (TBP). We found that UAF recognizes DNA using a hexameric histone-like scaffold with markedly different interactions compared with the nucleosome and the histone-fold-rich transcription factor IID (TFIID). In parallel, UAF positions TBP for Core Factor binding, which leads to Pol I recruitment, while sequestering it from DNA and Pol II/III-specific transcription factors. Our work thus reveals the structural basis of RNA Pol selection by a transcription factor. |
リンク | Sci Adv / PubMed:35442737 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.8 Å |
| 構造データ | EMDB-14428, PDB-7z0o: |
| 由来 |
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キーワード | TRANSCRIPTION / Transcription Factor / Nucleolus / Histone Fold |
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