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Structure paper

TitleModular assembly of the principal microtubule nucleator γ-TuRC.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 473, Year 2022
Publish dateJan 25, 2022
AuthorsMartin Würtz / Erik Zupa / Enrico S Atorino / Annett Neuner / Anna Böhler / Ariani S Rahadian / Bram J A Vermeulen / Giulia Tonon / Sebastian Eustermann / Elmar Schiebel / Stefan Pfeffer /
PubMed AbstractThe gamma-tubulin ring complex (γ-TuRC) is the principal microtubule nucleation template in vertebrates. Recent cryo-EM reconstructions visualized the intricate quaternary structure of the γ-TuRC, ...The gamma-tubulin ring complex (γ-TuRC) is the principal microtubule nucleation template in vertebrates. Recent cryo-EM reconstructions visualized the intricate quaternary structure of the γ-TuRC, containing more than thirty subunits, raising fundamental questions about γ-TuRC assembly and the role of actin as an integral part of the complex. Here, we reveal the structural mechanism underlying modular γ-TuRC assembly and identify a functional role of actin in microtubule nucleation. During γ-TuRC assembly, a GCP6-stabilized core comprising GCP2-3-4-5-4-6 is expanded by stepwise recruitment, selective stabilization and conformational locking of four pre-formed GCP2-GCP3 units. Formation of the lumenal bridge specifies incorporation of the terminal GCP2-GCP3 unit and thereby leads to closure of the γ-TuRC ring in a left-handed spiral configuration. Actin incorporation into the complex is not relevant for γ-TuRC assembly and structural integrity, but determines γ-TuRC geometry and is required for efficient microtubule nucleation and mitotic chromosome alignment in vivo.
External linksNat Commun / PubMed:35078983 / PubMed Central
MethodsEM (single particle)
Resolution5.32 - 16.1 Å
Structure data

14005

7qj0

EMDB-14005, PDB-7qj0:
Structure of recombinant human gamma-Tubulin Ring Complex 6-spoked assembly intermediate (spokes 7-12)
Method: EM (single particle) / Resolution: 5.32 Å

14006

7qj1

EMDB-14006, PDB-7qj1:
Structure of the recombinant human gamma-Tubulin Ring Complex 6-spoked assembly intermediate (spokes 7-12, homogeneous dataset)
Method: EM (single particle) / Resolution: 7.0 Å

14007

7qj2

EMDB-14007, PDB-7qj2:
Structure of recombinant human gamma-Tubulin Ring Complex 8-spoked assembly intermediate (spokes 5-12)
Method: EM (single particle) / Resolution: 8.6 Å

14008

7qj3

EMDB-14008, PDB-7qj3:
Structure of recombinant human gamma-Tubulin Ring Complex 8-spoked assembly intermediate (spokes 7-14)
Method: EM (single particle) / Resolution: 7.6 Å

14009

7qj4

EMDB-14009, PDB-7qj4:
Structure of recombinant human gamma-Tubulin Ring Complex 10-spoked assembly intermediate (spokes 5-14)
Method: EM (single particle) / Resolution: 9.0 Å

14010

7qj5

EMDB-14010, PDB-7qj5:
Structure of recombinant human gamma-Tubulin Ring Complex (spokes 1-14)
Method: EM (single particle) / Resolution: 8.7 Å

14011

7qj6

EMDB-14011, PDB-7qj6:
Structure of recombinant human gamma-Tubulin Ring Complex 10-spoked assembly intermediate (spokes 3-12, substoichiometric spokes 13-14)
Method: EM (single particle) / Resolution: 7.8 Å

14012

7qj7

EMDB-14012, PDB-7qj7:
Structure of recombinant human gamma-Tubulin Ring Complex 12-spoked assembly intermediate (spokes 1-12 substoichiometric spokes 13-14)
Method: EM (single particle) / Resolution: 8.7 Å

14013

7qj8

EMDB-14013, PDB-7qj8:
Structure of recombinant human gamma-Tubulin Ring Complex 12-spoked assembly intermediate (spokes 3-14, substoichiometric spokes 1-2)
Method: EM (single particle) / Resolution: 8.7 Å

14014

7qj9

EMDB-14014, PDB-7qj9:
Structure of recombinant human gamma-Tubulin Ring Complex 10-spoked assembly intermediate (spokes 3-12, homogeneous dataset)
Method: EM (single particle) / Resolution: 8.1 Å

14015

7qja

EMDB-14015, PDB-7qja:
Structure of recombinant human gamma-Tubulin Ring Complex 12-spoked assembly intermediate (spokes 1-12, homogeneous dataset)
Method: EM (single particle) / Resolution: 9.2 Å

14016

7qjb

EMDB-14016, PDB-7qjb:
Structure of recombinant human gamma-Tubulin Ring Complex 12-spoked assembly intermediate (spokes 3-14, homogeneous dataset)
Method: EM (single particle) / Resolution: 9.2 Å

14017

7qjc

EMDB-14017, PDB-7qjc:
Structure of recombinant human gamma-Tubulin Ring Complex (spokes 1-14, homogeneous dataset)
Method: EM (single particle) / Resolution: 16.1 Å

14018

7qjd

EMDB-14018, PDB-7qjd:
Structure of recombinant human gamma-Tubulin Ring Complex without actin
Method: EM (single particle) / Resolution: 7.1 Å

14019

7qje

EMDB-14019, PDB-7qje:
Structure of recombinant human gamma-Tubulin Ring Complex 4-spoked assembly intermediate (spokes 9-12)
Method: EM (single particle) / Resolution: 7.8 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsCYTOSOLIC PROTEIN / Assembly / Intermediate / Complex

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