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TitleStructural Analysis of Botulinum Neurotoxins Type B and E by Cryo-EM.
Journal, issue, pagesToxins (Basel), Vol. 14, Issue 1, Year 2021
Publish dateDec 23, 2021
AuthorsSara Košenina / Markel Martínez-Carranza / Jonathan R Davies / Geoffrey Masuyer / Pål Stenmark /
PubMed AbstractBotulinum neurotoxins (BoNTs) are the causative agents of a potentially lethal paralytic disease targeting cholinergic nerve terminals. Multiple BoNT serotypes exist, with types A, B and E being the ...Botulinum neurotoxins (BoNTs) are the causative agents of a potentially lethal paralytic disease targeting cholinergic nerve terminals. Multiple BoNT serotypes exist, with types A, B and E being the main cause of human botulism. Their extreme toxicity has been exploited for cosmetic and therapeutic uses to treat a wide range of neuromuscular disorders. Although naturally occurring BoNT types share a common end effect, their activity varies significantly based on the neuronal cell-surface receptors and intracellular SNARE substrates they target. These properties are the result of structural variations that have traditionally been studied using biophysical methods such as X-ray crystallography. Here, we determined the first structures of botulinum neurotoxins using single-particle cryogenic electron microscopy. The maps obtained at 3.6 and 3.7 Å for BoNT/B and /E, respectively, highlight the subtle structural dynamism between domains, and of the binding domain in particular. This study demonstrates how the recent advances made in the field of single-particle electron microscopy can be applied to bacterial toxins of clinical relevance and the botulinum neurotoxin family in particular.
External linksToxins (Basel) / PubMed:35050991 / PubMed Central
MethodsEM (single particle)
Resolution3.6 - 3.7 Å
Structure data

13946

7qfp

EMDB-13946, PDB-7qfp:
Cryo-EM structure of Botulinum neurotoxin serotype E
Method: EM (single particle) / Resolution: 3.7 Å

13947

7qfq

EMDB-13947, PDB-7qfq:
Cryo-EM structure of Botulinum neurotoxin serotype B
Method: EM (single particle) / Resolution: 3.6 Å

Source
  • clostridium botulinum (bacteria)
KeywordsTOXIN / clostridium botulinum / neurotoxin

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