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TitleSnapshots of nuclear pore complexes in action captured by cryo-electron tomography.
Journal, issue, pagesNature, Vol. 449, Issue 7162, Page 611-615, Year 2007
Publish dateOct 4, 2007
AuthorsMartin Beck / Vladan Lucić / Friedrich Förster / Wolfgang Baumeister / Ohad Medalia /
PubMed AbstractNuclear pore complexes reside in the nuclear envelope of eukaryotic cells and mediate the nucleocytoplasmic exchange of macromolecules. Traffic is regulated by mobile transport receptors that target ...Nuclear pore complexes reside in the nuclear envelope of eukaryotic cells and mediate the nucleocytoplasmic exchange of macromolecules. Traffic is regulated by mobile transport receptors that target their cargo to the central translocation channel, where phenylalanine-glycine-rich repeats serve as binding sites. The structural analysis of the nuclear pore is a formidable challenge given its size, its location in a membranous environment and its dynamic nature. Here we have used cryo-electron tomography to study the structure of nuclear pore complexes in their functional environment, that is, in intact nuclei of Dictyostelium discoideum. A new image-processing strategy compensating for deviations of the asymmetric units (protomers) from a perfect eight-fold symmetry enabled us to refine the structure and to identify new features. Furthermore, the superposition of a large number of tomograms taken in the presence of cargo, which was rendered visible by gold nanoparticles, has yielded a map outlining the trajectories of import cargo. Finally, we have performed single-molecule Monte Carlo simulations of nuclear import to interpret the experimentally observed cargo distribution in the light of existing models for nuclear import.
External linksNature / PubMed:17851530
MethodsEM (subtomogram averaging)
Resolution58.0 Å
Structure data

EMDB-1394:
Snapshots of nuclear pore complexes in action captured by cryo-electron tomography.
Method: EM (subtomogram averaging) / Resolution: 58.0 Å

Source
  • Dictyostelium discoideum (eukaryote)

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