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TitleStructural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 6992, Year 2021
Publish dateNov 30, 2021
AuthorsGuillermo Abascal-Palacios / Laura Jochem / Carlos Pla-Prats / Fabienne Beuron / Alessandro Vannini /
PubMed AbstractRetrotransposons are endogenous elements that have the ability to mobilise their DNA between different locations in the host genome. The Ty3 retrotransposon integrates with an exquisite specificity ...Retrotransposons are endogenous elements that have the ability to mobilise their DNA between different locations in the host genome. The Ty3 retrotransposon integrates with an exquisite specificity in a narrow window upstream of RNA Polymerase (Pol) III-transcribed genes, representing a paradigm for harmless targeted integration. Here we present the cryo-EM reconstruction at 4.0 Å of an active Ty3 strand transfer complex bound to TFIIIB transcription factor and a tRNA gene. The structure unravels the molecular mechanisms underlying Ty3 targeting specificity at Pol III-transcribed genes and sheds light into the architecture of retrotransposon machinery during integration. Ty3 intasome contacts a region of TBP, a subunit of TFIIIB, which is blocked by NC2 transcription regulator in RNA Pol II-transcribed genes. A newly-identified chromodomain on Ty3 integrase interacts with TFIIIB and the tRNA gene, defining with extreme precision the integration site position.
External linksNat Commun / PubMed:34848735 / PubMed Central
MethodsEM (single particle)
Resolution3.98 Å
Structure data

13831

7q5b

EMDB-13831, PDB-7q5b:
Cryo-EM structure of Ty3 retrotransposon targeting a TFIIIB-bound tRNA gene
Method: EM (single particle) / Resolution: 3.98 Å

Source
  • saccharomyces cerevisiae s288c (yeast)
KeywordsDNA BINDING PROTEIN / Transcription / RNA Pol III / Ty3 Retrotransposon / Intasome

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