Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Transcription factors modulate RNA polymerase conformational equilibrium.
Journal, issue, pages	Nat Commun, Vol. 13, Issue 1, Page 1546, Year 2022
Publish date	Mar 22, 2022
Authors	Chengjin Zhu / Xieyang Guo / Philippe Dumas / Maria Takacs / Mo'men Abdelkareem / Arnaud Vanden Broeck / Charlotte Saint-André / Gabor Papai / Corinne Crucifix / Julio Ortiz / Albert Weixlbaumer /
PubMed Abstract	RNA polymerase (RNAP) frequently pauses during the transcription of DNA to RNA to regulate gene expression. Transcription factors NusA and NusG modulate pausing, have opposing roles, but can bind ...RNA polymerase (RNAP) frequently pauses during the transcription of DNA to RNA to regulate gene expression. Transcription factors NusA and NusG modulate pausing, have opposing roles, but can bind RNAP simultaneously. Here we report cryo-EM reconstructions of Escherichia coli RNAP bound to NusG, or NusA, or both. RNAP conformational changes, referred to as swivelling, correlate with transcriptional pausing. NusA facilitates RNAP swivelling to further increase pausing, while NusG counteracts this role. Their structural effects are consistent with biochemical results on two categories of transcriptional pauses. In addition, the structures suggest a cooperative mechanism of NusA and NusG during Rho-mediated transcription termination. Our results provide a structural rationale for the stochastic nature of pausing and termination and how NusA and NusG can modulate it.
External links	Nat Commun / PubMed:35318334 / PubMed Central
Methods	EM (single particle)
Resolution	3.8 - 4.5 Å
Structure data	13706 7py0 EMDB-13706, PDB-7py0: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusG (NusG-EC in more-swiveled conformation) Method: EM (single particle) / Resolution: 4.5 Å 13707 7py1 EMDB-13707, PDB-7py1: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusG (the consensus NusG-EC) Method: EM (single particle) / Resolution: 3.8 Å 13709 7py3 EMDB-13709, PDB-7py3: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA (the consensus NusA-EC) Method: EM (single particle) / Resolution: 3.8 Å 13713 7py5 EMDB-13713, PDB-7py5: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA and NusG (the consensus NusA-NusG-EC) Method: EM (single particle) / Resolution: 3.9 Å 13714 7py6 EMDB-13714, PDB-7py6: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA and NusG (NusA and NusG elongation complex in less-swiveled conformation) Method: EM (single particle) / Resolution: 4.1 Å 13715 7py7 EMDB-13715, PDB-7py7: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA and NusG (NusA and NusG elongation complex in more-swiveled conformation) Method: EM (single particle) / Resolution: 4.1 Å 13716 7py8 EMDB-13716, PDB-7py8: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusG (NusG-EC in less-swiveled conformation) Method: EM (single particle) / Resolution: 3.8 Å 13717 7pyj EMDB-13717, PDB-7pyj: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA (NusA elongation complex in less-swiveled conformation) Method: EM (single particle) / Resolution: 4.2 Å 13718 7pyk EMDB-13718, PDB-7pyk: CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA (NusA elongation complex in more-swiveled conformation) Method: EM (single particle) / Resolution: 4.1 Å 13745 7q0j EMDB-13745, PDB-7q0j: RNA polymerase elongation complex in more-swiveled conformation Method: EM (single particle) / Resolution: 4.3 Å 13746 7q0k EMDB-13746, PDB-7q0k: RNA polymerase elongation complex in less-swiveled conformation Method: EM (single particle) / Resolution: 4.0 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry
Source	escherichia coli (E. coli) escherichia coli ms 115-1 (bacteria)
Keywords	TRANSCRIPTION / NusG / transcription elongation / cryo-EM / NusA / NusA and NusG / RNAP transcription elongation