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TitleMg-dependent conformational equilibria in CorA and an integrated view on transport regulation.
Journal, issue, pagesElife, Vol. 11, Year 2022
Publish dateFeb 7, 2022
AuthorsNicolai Tidemand Johansen / Marta Bonaccorsi / Tone Bengtsen / Andreas Haahr Larsen / Frederik Grønbæk Tidemand / Martin Cramer Pedersen / Pie Huda / Jens Berndtsson / Tamim Darwish / Nageshewar Rao Yepuri / Anne Martel / Thomas Günther Pomorski / Andrea Bertarello / Mark Sansom / Mikaela Rapp / Ramon Crehuet / Tobias Schubeis / Kresten Lindorff-Larsen / Guido Pintacuda / Lise Arleth /
PubMed AbstractThe CorA family of proteins regulates the homeostasis of divalent metal ions in many bacteria, archaea, and eukaryotic mitochondria, making it an important target in the investigation of the ...The CorA family of proteins regulates the homeostasis of divalent metal ions in many bacteria, archaea, and eukaryotic mitochondria, making it an important target in the investigation of the mechanisms of transport and its functional regulation. Although numerous structures of open and closed channels are now available for the CorA family, the mechanism of the transport regulation remains elusive. Here, we investigated the conformational distribution and associated dynamic behaviour of the pentameric Mg channel CorA at room temperature using small-angle neutron scattering (SANS) in combination with molecular dynamics (MD) simulations and solid-state nuclear magnetic resonance spectroscopy (NMR). We find that neither the Mg-bound closed structure nor the Mg-free open forms are sufficient to explain the average conformation of CorA. Our data support the presence of conformational equilibria between multiple states, and we further find a variation in the behaviour of the backbone dynamics with and without Mg. We propose that CorA must be in a dynamic equilibrium between different non-conducting states, both symmetric and asymmetric, regardless of bound Mg but that conducting states become more populated in Mg-free conditions. These properties are regulated by backbone dynamics and are key to understanding the functional regulation of CorA.
External linksElife / PubMed:35129435 / PubMed Central
MethodsEM (single particle)
Resolution15.0 - 16.0 Å
Structure data

EMDB-13326:
T. maritima CorA in DDM micelles without Mg2+ bound in D2O
Method: EM (single particle) / Resolution: 15.0 Å

EMDB-13327:
T. maritima CorA in DDM micelles with Mg2+ bound in D2O
Method: EM (single particle) / Resolution: 16.0 Å

Source
  • Thermotoga maritima (bacteria)

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