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TitleDirect visualization of the putative portal in the Kaposi's sarcoma-associated herpesvirus capsid by cryoelectron tomography.
Journal, issue, pagesJ Virol, Vol. 81, Issue 7, Page 3640-3644, Year 2007
Publish dateJan 10, 2007
AuthorsBinbin Deng / Christine M O'Connor / Dean H Kedes / Z Hong Zhou /
PubMed AbstractGenetic and biochemical studies have suggested the existence of a bacteriophage-like, DNA-packaging/ejecting portal complex in herpesviruses capsids, but its arrangement remained unknown. Here, we ...Genetic and biochemical studies have suggested the existence of a bacteriophage-like, DNA-packaging/ejecting portal complex in herpesviruses capsids, but its arrangement remained unknown. Here, we report the first visualization of a unique vertex in the Kaposi's sarcoma-associated herpesvirus (KSHV) capsid by cryoelectron tomography, thus providing direct structural evidence for the existence of a portal complex in a gammaherpesvirus. This putative KSHV portal is an internally localized, umbilicated structure and lacks all of the external machineries characteristic of portals in DNA bacteriophages.
External linksJ Virol / PubMed:17215290 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution40.0 Å
Structure data

EMDB-1320:
Direct visualization of the putative portal in the Kaposi's sarcoma-associated herpesvirus capsid by cryoelectron tomography.
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

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