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TitleAnalysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
Journal, issue, pagesPLoS One, Vol. 16, Issue 11, Page e0252497, Year 2021
Publish dateNov 23, 2021
AuthorsGisela Pöll / Michael Pilsl / Joachim Griesenbeck / Herbert Tschochner / Philipp Milkereit /
PubMed AbstractIn yeast and human cells many of the ribosomal proteins (r-proteins) are required for the stabilisation and productive processing of rRNA precursors. Functional coupling of r-protein assembly with ...In yeast and human cells many of the ribosomal proteins (r-proteins) are required for the stabilisation and productive processing of rRNA precursors. Functional coupling of r-protein assembly with the stabilisation and maturation of subunit precursors potentially promotes the production of ribosomes with defined composition. To further decipher mechanisms of such an intrinsic quality control pathway we analysed here the contribution of three yeast large ribosomal subunit r-proteins rpL2 (uL2), rpL25 (uL23) and rpL34 (eL34) for intermediate nuclear subunit folding steps. Structure models obtained from single particle cryo-electron microscopy analyses provided evidence for specific and hierarchic effects on the stable positioning and remodelling of large ribosomal subunit domains. Based on these structural and previous biochemical data we discuss possible mechanisms of r-protein dependent hierarchic domain arrangement and the resulting impact on the stability of misassembled subunits.
External linksPLoS One / PubMed:34813592 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 4.72 Å
Structure data

12866

7of1

EMDB-12866, PDB-7of1:
Nog1-TAP associated immature ribosomal particle population A from S. cerevisiae
Method: EM (single particle) / Resolution: 3.1 Å

12892

7oh3

EMDB-12892, PDB-7oh3:
Nog1-TAP associated immature ribosomal particle population B from S. cerevisiae
Method: EM (single particle) / Resolution: 3.4 Å

12904

7ohp

EMDB-12904, PDB-7ohp:
Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL25 expression shut down, population A
Method: EM (single particle) / Resolution: 3.9 Å

12905

7ohq

EMDB-12905, PDB-7ohq:
Nog1-TAP associated immature ribosomal particle population C from S. cerevisiae
Method: EM (single particle) / Resolution: 3.1 Å

12906

7ohr

EMDB-12906, PDB-7ohr:
Nog1-TAP associated immature ribosomal particle population E from S. cerevisiae
Method: EM (single particle) / Resolution: 4.72 Å

12907

7ohs

EMDB-12907, PDB-7ohs:
Nog1-TAP associated immature ribosomal particle population F from S. cerevisiae
Method: EM (single particle) / Resolution: 4.38 Å

12908

7oht

EMDB-12908, PDB-7oht:
Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL2 expression shut down, population A
Method: EM (single particle) / Resolution: 4.7 Å

12909

7ohu

EMDB-12909, PDB-7ohu:
Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL2 expression shut down, population B
Method: EM (single particle) / Resolution: 3.7 Å

12910

7ohv

EMDB-12910, PDB-7ohv:
Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL2 expression shut down, population C
Method: EM (single particle) / Resolution: 3.9 Å

12911

7ohw

EMDB-12911, PDB-7ohw:
Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL25 expression shut down, population B
Method: EM (single particle) / Resolution: 3.5 Å

12912

7ohx

EMDB-12912, PDB-7ohx:
Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL34 expression shut down, population A
Method: EM (single particle) / Resolution: 3.3 Å

12913

7ohy

EMDB-12913, PDB-7ohy:
Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL34 expression shut down, population B
Method: EM (single particle) / Resolution: 3.9 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae s288c (yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsRIBOSOME / ribosomal assembly state

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