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TitleStructure of the bacteriophage PhiKZ non-virion RNA polymerase.
Journal, issue, pagesNucleic Acids Res, Vol. 49, Issue 13, Page 7732-7739, Year 2021
Publish dateJul 21, 2021
AuthorsNatàlia deYMartín Garrido / Mariia Orekhova / Yuen Ting Emilie Lai Wan Loong / Anna Litvinova / Kailash Ramlaul / Tatyana Artamonova / Alexei S Melnikov / Pavel Serdobintsev / Christopher H S Aylett / Maria Yakunina /
PubMed AbstractBacteriophage ΦKZ (PhiKZ) is the archetype of a family of massive bacterial viruses. It is considered to have therapeutic potential as its host, Pseudomonas aeruginosa, is an opportunistic, ...Bacteriophage ΦKZ (PhiKZ) is the archetype of a family of massive bacterial viruses. It is considered to have therapeutic potential as its host, Pseudomonas aeruginosa, is an opportunistic, intrinsically antibiotic resistant, pathogen that kills tens of thousands worldwide each year. ΦKZ is an incredibly interesting virus, expressing many systems that the host already possesses. On infection, it forms a 'nucleus', erecting a barrier around its genome to exclude host endonucleases and CRISPR-Cas systems. ΦKZ infection is independent of the host transcriptional apparatus. It expresses two different multi-subunit RNA polymerases (RNAPs): the virion RNAP (vRNAP) is injected with the viral DNA during infection to transcribe early genes, including those encoding the non-virion RNAP (nvRNAP), which transcribes all further genes. ΦKZ nvRNAP is formed by four polypeptides thought to represent homologues of the eubacterial β/β' subunits, and a fifth with unclear homology, but essential for transcription. We have resolved the structure of ΦKZ nvRNAP to better than 3.0 Å, shedding light on its assembly, homology, and the biological role of the fifth subunit: it is an embedded, integral member of the complex, the position, structural homology and biochemical role of which imply that it has evolved from an ancestral homologue to σ-factor.
External linksNucleic Acids Res / PubMed:34181731 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.3 Å
Structure data

12885

7ogp

EMDB-12885, PDB-7ogp:
Structure of the apo-state of the bacteriophage PhiKZ non-virion RNA polymerase - class including clamp
Method: EM (single particle) / Resolution: 3.3 Å

12886

7ogr

EMDB-12886, PDB-7ogr:
Structure of the apo-state of the bacteriophage PhiKZ non-virion RNA polymerase
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • Pseudomonas virus phiKZ
  • pseudomonas phage phikz (virus)
  • Pseudomonas phage PA7 (virus)
KeywordsTRANSCRIPTION / RNA polymerase / PhiKZ / non-virion

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