EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conformational Plasticity of Hepatitis B Core Protein Spikes Promotes Peptide Binding Independent of the Secretion Phenotype.
ジャーナル・号・ページ	Microorganisms, Vol. 9, Issue 5, Year 2021
掲載日	2021年4月29日
著者	Cihan Makbul / Vladimir Khayenko / Hans Michael Maric / Bettina Böttcher /
PubMed 要旨	Hepatitis B virus is a major human pathogen, which forms enveloped virus particles. During viral maturation, membrane-bound hepatitis B surface proteins package hepatitis B core protein capsids. This ...Hepatitis B virus is a major human pathogen, which forms enveloped virus particles. During viral maturation, membrane-bound hepatitis B surface proteins package hepatitis B core protein capsids. This process is intercepted by certain peptides with an "LLGRMKG" motif that binds to the capsids at the tips of dimeric spikes. With microcalorimetry, electron cryo microscopy and peptide microarray-based screens, we have characterized the structural and thermodynamic properties of peptide binding to hepatitis B core protein capsids with different secretion phenotypes. The peptide "GSLLGRMKGA" binds weakly to hepatitis B core protein capsids and mutant capsids with a premature (F97L) or low-secretion phenotype (L60V and P5T). With electron cryo microscopy, we provide novel structures for L60V and P5T and demonstrate that binding occurs at the tips of the spikes at the dimer interface, splaying the helices apart independent of the secretion phenotype. Peptide array screening identifies "SLLGRM" as the core binding motif. This shortened motif binds only to one of the two spikes in the asymmetric unit of the capsid and induces a much smaller conformational change. Altogether, these comprehensive studies suggest that the tips of the spikes act as an autonomous binding platform that is unaffected by mutations that affect secretion phenotypes.
リンク	Microorganisms / PubMed:33946808 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 3.2 Å
構造データ	12810 7oco EMDB-12810, PDB-7oco: Hepatitis B core protein -low secretion phenotype L60V 手法: EM (単粒子) / 解像度: 3.2 Å 12815 7ocw EMDB-12815, PDB-7ocw: Hepatitis B core protein -low secretion phenotype P5T 手法: EM (単粒子) / 解像度: 3.2 Å 12819 7od4 EMDB-12819, PDB-7od4: Hepatitis B core protein. 手法: EM (単粒子) / 解像度: 2.8 Å 12820 7od6 EMDB-12820, PDB-7od6: Hepatitis B core protein + GSLLGRMKGA 手法: EM (単粒子) / 解像度: 3.0 Å 12821 7od7 EMDB-12821, PDB-7od7: Hepatitis B core protein + SLLGRM 手法: EM (単粒子) / 解像度: 2.8 Å 12822 7od8 EMDB-12822, PDB-7od8: Hepatitis B core Protein mutant L60V + GSLLGRMKGA 手法: EM (単粒子) / 解像度: 3.0 Å 12823 7oew EMDB-12823: Hepatitis B core protein capsid, mutant F97L with bound MHRSLLGRMKGA PDB-7oew: Hepatitis B core protein mutant F97L with bound MHRSLLGRMKGA 手法: EM (単粒子) / 解像度: 2.9 Å 12824 7oev EMDB-12824: Hepatitis B core Protein, F97L - premature secretion mutant with bound GSLLGRMKGA PDB-7oev: Hepatitis B core protein mutant F97L with bound GSLLGRMKGA 手法: EM (単粒子) / 解像度: 3.1 Å 12825 7oen EMDB-12825: Hepatitis B core protein with low secretion phenotype (P5T) and bound GSLLGRMKGA PDB-7oen: Hepatitis B core protein mutant P5T with bound GSLLGRMKGA 手法: EM (単粒子) / 解像度: 3.2 Å
由来	hepatitis b virus genotype d subtype ayw (isolate france/tiollais/1979) (B型肝炎ウイルス) hepatitis b virus (B 型肝炎ウイルス) synthetic construct (人工物)
キーワード	VIRUS LIKE PARTICLE (ウイルス様粒子) / low secretion phenotype / L60V / Hepatitis B core protein / P5T / inhibitory peptide (酵素阻害剤) / Hepatitis B virus (B型肝炎ウイルス) / peptide inhibitor / premature envelopment mutant / F97L / GSLLGRMKGA / MHRSLLGRMKGA