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Structure paper

TitleStructural basis for late maturation steps of the human mitoribosomal large subunit.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 3673, Year 2021
Publish dateJun 16, 2021
AuthorsMiriam Cipullo / Genís Valentín Gesé / Anas Khawaja / B Martin Hällberg / Joanna Rorbach /
PubMed AbstractMitochondrial ribosomes (mitoribosomes) synthesize a critical set of proteins essential for oxidative phosphorylation. Therefore, mitoribosomal function is vital to the cellular energy supply. ...Mitochondrial ribosomes (mitoribosomes) synthesize a critical set of proteins essential for oxidative phosphorylation. Therefore, mitoribosomal function is vital to the cellular energy supply. Mitoribosome biogenesis follows distinct molecular pathways that remain poorly understood. Here, we determine the cryo-EM structures of mitoribosomes isolated from human cell lines with either depleted or overexpressed mitoribosome assembly factor GTPBP5, allowing us to capture consecutive steps during mitoribosomal large subunit (mt-LSU) biogenesis. Our structures provide essential insights into the last steps of 16S rRNA folding, methylation and peptidyl transferase centre (PTC) completion, which require the coordinated action of nine assembly factors. We show that mammalian-specific MTERF4 contributes to the folding of 16S rRNA, allowing 16 S rRNA methylation by MRM2, while GTPBP5 and NSUN4 promote fine-tuning rRNA rearrangements leading to PTC formation. Moreover, our data reveal an unexpected involvement of the elongation factor mtEF-Tu in mt-LSU assembly, where mtEF-Tu interacts with GTPBP5, similar to its interaction with tRNA during translational elongation.
External linksNat Commun / PubMed:34135318 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 3.2 Å
Structure data

12763

7o9k

EMDB-12763, PDB-7o9k:
Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1, the MALSU module, GTPBP5 and mtEF-Tu
Method: EM (single particle) / Resolution: 3.1 Å

12764

7o9m

EMDB-12764, PDB-7o9m:
Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1 and the MALSU module
Method: EM (single particle) / Resolution: 2.6 Å

EMDB-12767:
Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1, the MALSU module, GTPBP5 and mtEF-TU (MTG1-loaded subset)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-12768:
Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1, the MALSU module, GTPBP5 and mtEF-TU (mtEF-Tu-loaded subset)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-12769:
Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2, MTG1 and the MALSU module (MTG1-loaded subset)
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-12770:
Human mitochondrial ribosome large subunit assembly intermediate with MTERF4-NSUN4, MRM2 and the MALSU module
Method: EM (single particle) / Resolution: 2.8 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-SAM:
S-ADENOSYLMETHIONINE / S-Adenosyl methionine

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE / S-Adenosyl-L-homocysteine

ChemComp-PNS:
4'-PHOSPHOPANTETHEINE / Phosphopantetheine

Source
  • homo sapiens (human)
  • Human (human)
KeywordsRIBOSOME / Mitochondria / GTPase / Ribosome assembly intermediate

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