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TitleOrganization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy.
Journal, issue, pagesMol Cell, Vol. 24, Issue 2, Page 267-278, Year 2006
Publish dateOct 20, 2006
AuthorsBjoern Sander / Monika M Golas / Evgeny M Makarov / Hero Brahms / Berthold Kastner / Reinhard Lührmann / Holger Stark /
PubMed AbstractIn eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic ...In eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic macromolecular machine. U4/U6.U5 tri-snRNP is the largest and most conserved building block of the spliceosome. By 3D electron cryomicroscopy and labeling, the exon-aligning U5 snRNA loop I is localized at the center of the tetrahedrally shaped tri-snRNP reconstructed to approximately 2.1 nm resolution in vitrified ice. Independent 3D reconstructions of its subunits, U4/U6 and U5 snRNPs, show how U4/U6 and U5 combine to form tri-snRNP and, together with labeling experiments, indicate a close proximity of the spliceosomal core components U5 snRNA loop I and U4/U6 at the center of tri-snRNP. We suggest that this central tri-snRNP region may be the site to which the prespliceosomal U2 snRNA has to approach closely during formation of the catalytic core of the spliceosome.
External linksMol Cell / PubMed:17052460
MethodsEM (single particle)
Resolution24.0 - 40.0 Å
Structure data

EMDB-1257:
Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy.
Method: EM (single particle) / Resolution: 24.0 Å

EMDB-1258:
Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy.
Method: EM (single particle) / Resolution: 32.0 Å

EMDB-1259:
Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy.
Method: EM (single particle) / Resolution: 40.0 Å

Source
  • Homo sapiens (human)

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