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-Structure paper
Title | Multiple distinct assemblies reveal conformational flexibility in the small heat shock protein Hsp26. |
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Journal, issue, pages | Structure, Vol. 14, Issue 7, Page 1197-1204, Year 2006 |
Publish date | Sep 15, 2006 |
Authors | Helen E White / Elena V Orlova / Shaoxia Chen / Luchun Wang / Athanasios Ignatiou / Brent Gowen / Thusnelda Stromer / Titus M Franzmann / Martin Haslbeck / Johannes Buchner / Helen R Saibil / |
PubMed Abstract | Small heat shock proteins are a superfamily of molecular chaperones that suppress protein aggregation and provide protection from cell stress. A key issue for understanding their action is to define ...Small heat shock proteins are a superfamily of molecular chaperones that suppress protein aggregation and provide protection from cell stress. A key issue for understanding their action is to define the interactions of subunit domains in these oligomeric assemblies. Cryo-electron microscopy of yeast Hsp26 reveals two distinct forms, each comprising 24 subunits arranged in a porous shell with tetrahedral symmetry. The subunits form elongated, asymmetric dimers that assemble via trimeric contacts. Modifications of both termini cause rearrangements that yield a further four assemblies. Each subunit contains an N-terminal region, a globular middle domain, the alpha-crystallin domain, and a C-terminal tail. Twelve of the C termini form 3-fold assembly contacts which are inserted into the interior of the shell, while the other 12 C termini form contacts on the surface. Hinge points between the domains allow a variety of assembly contacts, providing the flexibility required for formation of supercomplexes with non-native proteins. |
External links | Structure / PubMed:16843901 |
Methods | EM (single particle) |
Resolution | 10.8 - 14.5 Å |
Structure data | EMDB-1221: Multiple distinct assemblies reveal conformational flexibility in the small heat shock protein Hsp26. EMDB-1226: Multiple distinct assemblies reveal conformational flexibility in the small heat shock protein Hsp26. EMDB-1227: EMDB-1228: EMDB-1229: EMDB-1230: |
Source |
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Keywords | CHAPERONE / alpha-crystallin / chaperones / heat shock proteins / single particle reconstruction |