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TitleDifferences in structure and hibernation mechanism highlight diversification of the microsporidian ribosome.
Journal, issue, pagesPLoS Biol, Vol. 18, Issue 10, Page e3000958, Year 2020
Publish dateOct 30, 2020
AuthorsKai Ehrenbolger / Nathan Jespersen / Himanshu Sharma / Yuliya Y Sokolova / Yuri S Tokarev / Charles R Vossbrinck / Jonas Barandun /
PubMed AbstractAssembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. In organisms operating under strict nutrient limitations, such as pathogenic ...Assembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of energy via ribosomal hibernation and recycling is critical. The mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. Here, we present the cryo-electron microscopy structure of the ribosome from Paranosema locustae spores, bound by the conserved eukaryotic hibernation and recycling factor Lso2. The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism. Although microsporidian ribosomes are highly compacted, the P. locustae ribosome retains several rRNA segments absent in other microsporidia, and represents an intermediate state of rRNA reduction. In one case, the near complete reduction of an expansion segment has resulted in a single bound nucleotide, which may act as an architectural co-factor to stabilize a protein-protein interface. The presented structure highlights the reductive evolution in these emerging pathogens and sheds light on a conserved mechanism for eukaryotic ribosome hibernation.
External linksPLoS Biol / PubMed:33125369 / PubMed Central
MethodsEM (single particle)
Resolution2.9 Å
Structure data

11437

6zu5

EMDB-11437, PDB-6zu5:
Structure of the Paranosema locustae ribosome in complex with Lso2
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM / Adenosine monophosphate

Source
  • paranosema locustae (fungus)
KeywordsRIBOSOME / Microsporidia / Pathogen / Hibernation / Genome Compaction

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