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TitleChemically induced protein cage assembly with programmable opening and cargo release.
Journal, issue, pagesSci Adv, Vol. 8, Issue 1, Page eabj9424, Year 2022
Publish dateJan 7, 2022
AuthorsIzabela Stupka / Yusuke Azuma / Artur P Biela / Motonori Imamura / Simon Scheuring / Elżbieta Pyza / Olga Woźnicka / Daniel P Maskell / Jonathan G Heddle /
PubMed AbstractEngineered protein cages are promising tools that can be customized for applications in medicine and nanotechnology. A major challenge is developing a straightforward strategy for endowing cages with ...Engineered protein cages are promising tools that can be customized for applications in medicine and nanotechnology. A major challenge is developing a straightforward strategy for endowing cages with bespoke, inducible disassembly. Such cages would allow release of encapsulated cargoes at desired timing and location. Here, we achieve such programmable disassembly using protein cages, in which the subunits are held together by different molecular cross-linkers. This modular system enables cage disassembly to be controlled in a condition-dependent manner. Structural details of the resulting cages were determined using cryo–electron microscopy, which allowed observation of bridging cross-linkers at intended positions. Triggered disassembly was demonstrated by high-speed atomic force microscopy and subsequent cargo release using an encapsulated Förster resonance energy transfer pair whose signal depends on the quaternary structure of the cage.
External linksSci Adv / PubMed:34985943 / PubMed Central
MethodsEM (single particle)
Resolution4.71 - 6.9 Å
Structure data

EMDB-11412:
Structure of right-handed protein cage consisting of 24 eleven-membered ring proteins held together by BMH cross linkers
Method: EM (single particle) / Resolution: 6.9 Å

EMDB-11413:
Structure of left-handed protein cage consisting of 24 eleven-membered ring proteins held together by BMH cross linkers
Method: EM (single particle) / Resolution: 6.8 Å

EMDB-11414:
Structure of right-handed protein cage consisting of 24 eleven-membered ring proteins held together by DTME cross linkers
Method: EM (single particle) / Resolution: 4.83 Å

EMDB-11415:
Structure of left-handed protein cage consisting of 24 eleven-membered ring proteins held together by DTME cross linkers
Method: EM (single particle) / Resolution: 4.71 Å

Source
  • Geobacillus stearothermophilus (bacteria)

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