Movie
Controller
Structure viewers
About Yorodumi Papers
+Search query
-Structure paper
| Title | Symmetric arrangement of mitochondria:plasma membrane contacts between adjacent photoreceptor cells regulated by Opa1. |
|---|---|
| Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 117, Issue 27, Page 15684-15693, Year 2020 |
| Publish date | Jul 7, 2020 |
 Authors | Ingrid P Meschede / Nicholas C Ovenden / Miguel C Seabra / Clare E Futter / Marcela Votruba / Michael E Cheetham / Thomas Burgoyne /   |
| PubMed Abstract | Mitochondria are known to play an essential role in photoreceptor function and survival that enables normal vision. Within photoreceptors, mitochondria are elongated and extend most of the inner- ...Mitochondria are known to play an essential role in photoreceptor function and survival that enables normal vision. Within photoreceptors, mitochondria are elongated and extend most of the inner-segment length, where they supply energy for protein synthesis and the phototransduction machinery in the outer segment, as well as acting as a calcium store. Here, we examined the arrangement of the mitochondria within the inner segment in detail using three-dimensional (3D) electron microscopy techniques and show they are tethered to the plasma membrane in a highly specialized arrangement. Remarkably, mitochondria and their cristae openings align with those of neighboring inner segments. The pathway by which photoreceptors meet their high energy demands is not fully understood. We propose this to be a mechanism to share metabolites and assist in maintaining homeostasis across the photoreceptor cell layer. In the extracellular space between photoreceptors, Müller glial processes were identified. Due to the often close proximity to the inner-segment mitochondria, they may, too, play a role in the inner-segment mitochondrial arrangement as well as metabolite shuttling. OPA1 is an important factor in mitochondrial homeostasis, including cristae remodeling; therefore, we examined the photoreceptors of a heterozygous knockout mouse model. The cristae structure in the photoreceptors was not greatly affected, but the mitochondria were enlarged and had reduced alignment to neighboring inner-segment mitochondria. This indicates the importance of key regulators in maintaining this specialized photoreceptor mitochondrial arrangement. |
 External links | Proc Natl Acad Sci U S A / PubMed:32571921 / PubMed Central |
| Methods | EM (tomography) |
| Structure data |  EMDB-10904:  EMDB-11125:  EMDB-11126:  EMDB-11147:  EMDB-11148: |
| Source |
|
Mus musculus (house mouse)