EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Symmetric arrangement of mitochondria:plasma membrane contacts between adjacent photoreceptor cells regulated by Opa1.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 117, Issue 27, Page 15684-15693, Year 2020
掲載日	2020年7月7日
著者	Ingrid P Meschede / Nicholas C Ovenden / Miguel C Seabra / Clare E Futter / Marcela Votruba / Michael E Cheetham / Thomas Burgoyne /
PubMed 要旨	Mitochondria are known to play an essential role in photoreceptor function and survival that enables normal vision. Within photoreceptors, mitochondria are elongated and extend most of the inner- ...Mitochondria are known to play an essential role in photoreceptor function and survival that enables normal vision. Within photoreceptors, mitochondria are elongated and extend most of the inner-segment length, where they supply energy for protein synthesis and the phototransduction machinery in the outer segment, as well as acting as a calcium store. Here, we examined the arrangement of the mitochondria within the inner segment in detail using three-dimensional (3D) electron microscopy techniques and show they are tethered to the plasma membrane in a highly specialized arrangement. Remarkably, mitochondria and their cristae openings align with those of neighboring inner segments. The pathway by which photoreceptors meet their high energy demands is not fully understood. We propose this to be a mechanism to share metabolites and assist in maintaining homeostasis across the photoreceptor cell layer. In the extracellular space between photoreceptors, Müller glial processes were identified. Due to the often close proximity to the inner-segment mitochondria, they may, too, play a role in the inner-segment mitochondrial arrangement as well as metabolite shuttling. OPA1 is an important factor in mitochondrial homeostasis, including cristae remodeling; therefore, we examined the photoreceptors of a heterozygous knockout mouse model. The cristae structure in the photoreceptors was not greatly affected, but the mitochondria were enlarged and had reduced alignment to neighboring inner-segment mitochondria. This indicates the importance of key regulators in maintaining this specialized photoreceptor mitochondrial arrangement.
リンク	Proc Natl Acad Sci U S A / PubMed:32571921 / PubMed Central
手法	EM (トモグラフィー)
構造データ	EMDB-10904: Electron tomography data to study mitochondria within mouse photoreceptor inner segments. 手法: EM (トモグラフィー) EMDB-11125: Alignment of mitochondria between neighbouring photoreceptor inner segments 手法: EM (トモグラフィー) EMDB-11126: Mouse Muller glial projection 手法: EM (トモグラフィー) EMDB-11147: Heterozygous Opa1 knockout mouse photoreceptor inner segment mitochondria 手法: EM (トモグラフィー) EMDB-11148: Control (Opa1 +/+) wild-type mouse photoreceptor inner segment mitochondria 手法: EM (トモグラフィー)
由来	Mus musculus (ハツカネズミ)