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TitleA quasi-atomic model of human adenovirus type 5 capsid.
Journal, issue, pagesEMBO J, Vol. 24, Issue 9, Page 1645-1654, Year 2005
Publish dateMay 4, 2005
AuthorsCéline M S Fabry / Manuel Rosa-Calatrava / James F Conway / Chloé Zubieta / Stephen Cusack / Rob W H Ruigrok / Guy Schoehn /
PubMed AbstractAdenoviruses infect a wide range of vertebrates including humans. Their icosahedral capsids are composed of three major proteins: the trimeric hexon forms the facets and the penton, a noncovalent ...Adenoviruses infect a wide range of vertebrates including humans. Their icosahedral capsids are composed of three major proteins: the trimeric hexon forms the facets and the penton, a noncovalent complex of the pentameric penton base and trimeric fibre proteins, is located at the 12 capsid vertices. Several proteins (IIIa, VI, VIII and IX) stabilise the capsid. We have obtained a 10 A resolution map of the human adenovirus 5 by image analysis from cryo-electron micrographs (cryoEMs). This map, in combination with the X-ray structures of the penton base and hexon, was used to build a quasi-atomic model of the arrangement of the two major capsid components and to analyse the hexon-hexon and hexon-penton interactions. The secondary proteins, notably VIII, were located by comparing cryoEM maps of native and pIX deletion mutant virions. Minor proteins IX and IIIa are located on the outside of the capsid, whereas protein VIII is organised with a T=2 lattice on the inner face of the capsid. The capsid organisation is compared with the known X-ray structure of bacteriophage PRD1.
External linksEMBO J / PubMed:15861131 / PubMed Central
MethodsEM (single particle)
Resolution10 - 22.0 Å
Structure data

EMDB-1111: A quasi-atomic model of human adenovirus type 5 capsid.
PDB-4v4u: The quasi-atomic model of Human Adenovirus type 5 capsid
Method: EM (single particle) / Resolution: 22.0 Å

EMDB-1112: A quasi-atomic model of human adenovirus type 5 capsid.
PDB-4v4u: The quasi-atomic model of Human Adenovirus type 5 capsid
Method: EM (single particle) / Resolution: 15.5 Å

EMDB-1113: A quasi-atomic model of human adenovirus type 5 capsid.
PDB-4v4u: The quasi-atomic model of Human Adenovirus type 5 capsid
Method: EM (single particle) / Resolution: 10.3 Å

Source
  • human adenovirus 2
  • human adenovirus type 5
  • human adenovirus type 2
KeywordsVIRUS / ADENOVIRUS / MINOR CAPSID PROTEIN / QUASI ATOMIC

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