EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	In-cell architecture of an actively transcribing-translating expressome.
ジャーナル・号・ページ	Science, Vol. 369, Issue 6503, Page 554-557, Year 2020
掲載日	2020年7月31日
著者	Francis J O'Reilly / Liang Xue / Andrea Graziadei / Ludwig Sinn / Swantje Lenz / Dimitry Tegunov / Cedric Blötz / Neil Singh / Wim J H Hagen / Patrick Cramer / Jörg Stülke / Julia Mahamid / Juri Rappsilber /
PubMed 要旨	Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an integrative in-cell structural approach using the ...Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an integrative in-cell structural approach using the genome-reduced human pathogen We combined whole-cell cross-linking mass spectrometry, cellular cryo-electron tomography, and integrative modeling to determine an in-cell architecture of a transcribing and translating expressome at subnanometer resolution. The expressome comprises RNA polymerase (RNAP), the ribosome, and the transcription elongation factors NusG and NusA. We pinpointed NusA at the interface between a NusG-bound elongating RNAP and the ribosome and propose that it can mediate transcription-translation coupling. Translation inhibition dissociated the expressome, whereas transcription inhibition stalled and rearranged it. Thus, the active expressome architecture requires both translation and transcription elongation within the cell.
リンク	Science / PubMed:32732422 / PubMed Central
手法	EM (サブトモグラム平均)
解像度	5.6 - 9.7 Å
構造データ	EMDB-10677: in-cell 70S ribosome from Mycoplasma pneumoniae 手法: EM (サブトモグラム平均) / 解像度: 5.6 Å EMDB-10678: in-cell elongating expressome from Mycoplasma pneumoniae with focused refinement on RNAP 手法: EM (サブトモグラム平均) / 解像度: 9.2 Å EMDB-10679: in-cell elongating expressome from Mycoplasma pneumoniae 手法: EM (サブトモグラム平均) / 解像度: 8.5 Å EMDB-10680: Multibody refinement of RNA polymerase-NusA body of Mycoplasma pneumoniae in-cell expressome 手法: EM (サブトモグラム平均) / 解像度: 9.7 Å EMDB-10681: Multibody refinement of 30S ribosome body of Mycoplasma pneumoniae in-cell expressome 手法: EM (サブトモグラム平均) / 解像度: 8.0 Å EMDB-10682: Multibody refinement of 50S ribosome body of Mycoplasma pneumoniae in-cell expressome 手法: EM (サブトモグラム平均) / 解像度: 7.6 Å EMDB-10683: 70S ribosome from chloramphenicol-treated Mycoplasma pneumoniae cells 手法: EM (サブトモグラム平均) / 解像度: 6.5 Å EMDB-10684: in-cell stalled expressome from pseudouridimycin-treated Mycoplasma pneumoniae 手法: EM (サブトモグラム平均) / 解像度: 7.1 Å EMDB-10685: Multibody refinement of RNA polymerase body of pseudouridimycin-stalled Mycoplasma pneumoniae in-cell expressome 手法: EM (サブトモグラム平均) / 解像度: 8.1 Å EMDB-10686: Multibody refinement of 30S ribosome body of pseudouridimycin-stalled Mycoplasma pneumoniae in-cell expressome 手法: EM (サブトモグラム平均) / 解像度: 6.8 Å EMDB-10687: Multibody refinement of 50S ribosome body of pseudouridimycin-stalled Mycoplasma pneumoniae in-cell expressome 手法: EM (サブトモグラム平均) / 解像度: 6.2 Å
由来	Mycoplasma pneumoniae M129 (バクテリア)