Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	In-cell architecture of an actively transcribing-translating expressome.
Journal, issue, pages	Science, Vol. 369, Issue 6503, Page 554-557, Year 2020
Publish date	Jul 31, 2020
Authors	Francis J O'Reilly / Liang Xue / Andrea Graziadei / Ludwig Sinn / Swantje Lenz / Dimitry Tegunov / Cedric Blötz / Neil Singh / Wim J H Hagen / Patrick Cramer / Jörg Stülke / Julia Mahamid / Juri Rappsilber /
PubMed Abstract	Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an integrative in-cell structural approach using the ...Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an integrative in-cell structural approach using the genome-reduced human pathogen We combined whole-cell cross-linking mass spectrometry, cellular cryo-electron tomography, and integrative modeling to determine an in-cell architecture of a transcribing and translating expressome at subnanometer resolution. The expressome comprises RNA polymerase (RNAP), the ribosome, and the transcription elongation factors NusG and NusA. We pinpointed NusA at the interface between a NusG-bound elongating RNAP and the ribosome and propose that it can mediate transcription-translation coupling. Translation inhibition dissociated the expressome, whereas transcription inhibition stalled and rearranged it. Thus, the active expressome architecture requires both translation and transcription elongation within the cell.
External links	Science / PubMed:32732422 / PubMed Central
Methods	EM (subtomogram averaging)
Resolution	5.6 - 9.7 Å
Structure data	EMDB-10677: in-cell 70S ribosome from Mycoplasma pneumoniae Method: EM (subtomogram averaging) / Resolution: 5.6 Å EMDB-10678: in-cell elongating expressome from Mycoplasma pneumoniae with focused refinement on RNAP Method: EM (subtomogram averaging) / Resolution: 9.2 Å EMDB-10679: in-cell elongating expressome from Mycoplasma pneumoniae Method: EM (subtomogram averaging) / Resolution: 8.5 Å EMDB-10680: Multibody refinement of RNA polymerase-NusA body of Mycoplasma pneumoniae in-cell expressome Method: EM (subtomogram averaging) / Resolution: 9.7 Å EMDB-10681: Multibody refinement of 30S ribosome body of Mycoplasma pneumoniae in-cell expressome Method: EM (subtomogram averaging) / Resolution: 8.0 Å EMDB-10682: Multibody refinement of 50S ribosome body of Mycoplasma pneumoniae in-cell expressome Method: EM (subtomogram averaging) / Resolution: 7.6 Å EMDB-10683: 70S ribosome from chloramphenicol-treated Mycoplasma pneumoniae cells Method: EM (subtomogram averaging) / Resolution: 6.5 Å EMDB-10684: in-cell stalled expressome from pseudouridimycin-treated Mycoplasma pneumoniae Method: EM (subtomogram averaging) / Resolution: 7.1 Å EMDB-10685: Multibody refinement of RNA polymerase body of pseudouridimycin-stalled Mycoplasma pneumoniae in-cell expressome Method: EM (subtomogram averaging) / Resolution: 8.1 Å EMDB-10686: Multibody refinement of 30S ribosome body of pseudouridimycin-stalled Mycoplasma pneumoniae in-cell expressome Method: EM (subtomogram averaging) / Resolution: 6.8 Å EMDB-10687: Multibody refinement of 50S ribosome body of pseudouridimycin-stalled Mycoplasma pneumoniae in-cell expressome Method: EM (subtomogram averaging) / Resolution: 6.2 Å
Source	Mycoplasma pneumoniae M129 (bacteria)