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TitleTricalbin-Mediated Contact Sites Control ER Curvature to Maintain Plasma Membrane Integrity.
Journal, issue, pagesDev Cell, Vol. 51, Issue 4, Page 476-487.e7, Year 2019
Publish dateNov 18, 2019
AuthorsJavier Collado / Maria Kalemanov / Felix Campelo / Clélia Bourgoint / Ffion Thomas / Robbie Loewith / Antonio Martínez-Sánchez / Wolfgang Baumeister / Christopher J Stefan / Rubén Fernández-Busnadiego /
PubMed AbstractMembrane contact sites (MCS) between the endoplasmic reticulum (ER) and the plasma membrane (PM) play fundamental roles in all eukaryotic cells. ER-PM MCS are particularly abundant in Saccharomyces ...Membrane contact sites (MCS) between the endoplasmic reticulum (ER) and the plasma membrane (PM) play fundamental roles in all eukaryotic cells. ER-PM MCS are particularly abundant in Saccharomyces cerevisiae, where approximately half of the PM surface is covered by cortical ER (cER). Several proteins, including Ist2, Scs2/22, and Tcb1/2/3 are implicated in cER formation, but the specific roles of these molecules are poorly understood. Here, we use cryo-electron tomography to show that ER-PM tethers are key determinants of cER morphology. Notably, Tcb proteins (tricalbins) form peaks of extreme curvature on the cER membrane facing the PM. Combined modeling and functional assays suggest that Tcb-mediated cER peaks facilitate the transport of lipids between the cER and the PM, which is necessary to maintain PM integrity under heat stress. ER peaks were also present at other MCS, implying that membrane curvature enforcement may be a widespread mechanism to regulate MCS function.
External linksDev Cell / PubMed:31743662 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-10378:
Cryo-electron tomogram of cER-PM MCS in a heat-shocked WT S. cerevisiae cell
Method: EM (tomography)

EMDB-10379:
Cryo-electron tomogram of cER-PM MCS in a heat-shocked WT S. cerevisiae cell treated with a non-linear anisotropic filter
Method: EM (tomography)

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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