Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Antibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex.
Journal, issue, pages	Nat Commun, Vol. 11, Issue 1, Page 4948, Year 2020
Publish date	Oct 2, 2020
Authors	Marie Glavier / Dhenesh Puvanendran / Dimitri Salvador / Marion Decossas / Gilles Phan / Cyril Garnier / Elisa Frezza / Quentin Cece / Guy Schoehn / Martin Picard / Jean-Christophe Taveau / Laetitia Daury / Isabelle Broutin / Olivier Lambert /
PubMed Abstract	The tripartite multidrug efflux system MexAB-OprM is a major actor in Pseudomonas aeruginosa antibiotic resistance by exporting a large variety of antimicrobial compounds. Crystal structures of MexB ...The tripartite multidrug efflux system MexAB-OprM is a major actor in Pseudomonas aeruginosa antibiotic resistance by exporting a large variety of antimicrobial compounds. Crystal structures of MexB and of its Escherichia coli homolog AcrB had revealed asymmetric trimers depicting a directional drug pathway by a conformational interconversion (from Loose and Tight binding pockets to Open gate (LTO) for drug exit). It remains unclear how MexB acquires its LTO form. Here by performing functional and cryo-EM structural investigations of MexB at various stages of the assembly process, we unveil that MexB inserted in lipid membrane is not set for active transport because it displays an inactive LTC form with a Closed exit gate. In the tripartite complex, OprM and MexA form a corset-like platform that converts MexB into the active form. Our findings shed new light on the resistance nodulation cell division (RND) cognate partners which act as allosteric factors eliciting the functional drug extrusion.
External links	Nat Commun / PubMed:33009415 / PubMed Central
Methods	EM (single particle)
Resolution	3.2 - 4.5 Å
Structure data	10371 6t7s EMDB-10371: CryoEM structure of MexB in nanodisc PDB-6t7s: MexB structure solved by cryo-EM in nanodisc in absence of its protein partners Method: EM (single particle) / Resolution: 4.5 Å 10372 6ta5 EMDB-10372: CryoEM structure of MexAB-OprM in nanodisc PDB-6ta5: OprM-MexA complex from the MexAB-OprM Pseudomonas aeruginosa whole assembly reconstituted in nanodiscs Method: EM (single particle) / Resolution: 3.2 Å 10395 6ta6 EMDB-10395: MexA-MexB cryoEM map PDB-6ta6: MexAB assembly of the Pseudomonas MexAB-OprM efflux pump reconstituted in nanodiscs Method: EM (single particle) / Resolution: 3.2 Å
Source	pseudomonas aeruginosa (bacteria)
Keywords	ANTIMICROBIAL PROTEIN / transporter / efflux / proton motive force / bacterial resistance